大豆中2个编码 PR10蛋白新基因的克隆与分析

摘要

Two new soybean genes named Glyma09g04530 and Glyma15g15590 which encoding PR10 proteins were cloned from a SMV resistant cultivar Kefeng No .1.Glyma09g04530 and Glyma15g15590 have a 477 bp ORF and coding 158 amino acids .Sequence alignment and evolutionary tree clustering analysis showed that Gly-ma09g04530 and Glyma15g15590 are new PR10 protein genes in soybean .The genes of Glyma09g04530 and Gly-ma15 g15590 expressed in root ,stem and leave by using Real-time PCR analysis in soybean .Expression level of Gly-ma09g04530 and Glyma15g15590 were strongly induced and highly expressed in leaves of Kefeng No .1 after inocu-lation SMV .We speculate that Glyma09 g04530 and Glyma15 g15590 may be involved for getting resistance to SMV .%从抗大豆花叶病毒病（ SMV）大豆材料科丰1号中克隆到2个编码PR10蛋白的新基因Glyma09g05430和Glyma15g15509，Glyma09g04530和Glyma15g15590基因的ORF全长均为477 bp，编码158个氨基酸。序列比对与系统发生树分析结果表明：Glyma09g04530和Glyma15g15590是大豆中2个编码PR10蛋白的新基因。 Glyma09g04530和Glyma15g15590基因在大豆的根、茎及叶中均有表达；接种大豆SMV后，Glyma09g04530和Glyma15g15590基因在大豆科丰1号的叶片中的表达均被强烈诱导并高效表达，显示Glyma09g04530和Glyma15g15590基因与大豆SMV抗性密切相关。