白粉菌诱导的番茄叶片AD-cDNA文库的构建及初步分析

摘要

为了研究番茄白粉菌与番茄相互作用的分子机制，以白粉菌诱导的番茄叶片为材料，利用 SMART 技术构建了番茄的 AD-cDNA文库。该文库总库容量为3.77×1010 cfu，重组率达到96%，插入片段长400~2000 bp，平均长度约为1000 bp。随机挑取50个克隆进行测序，利用生物信息学软件进行初步分析，表明获得的文库质量较高。%In order to study the interaction between powdery mildew fungus and tomato,the AD-cDNA library of tomato leaves infected by Oidiun neolycopersici was constructed using SMART technology. The titer of the con-structed cDNA library was 3. 77 × 1010 cfu,and the recombination rate of the library was about 96%,the insert frag-ments ranged from 400 bp to 2 000 bp,and the average length of the library was about 1 000 bp. Fifty clones were randomly picked from the library for sequencing and functional analysis. The results indicated that the library was qualified for the study on the interaction between powdery mildew fungus and tomato.