津田芜菁泛素结合酶BrUBC11基因的克隆及表达分析

摘要

In order to elucidate the expression feature of UBC11 in Tsuda,cDNA of UBC11 gene was isolated from this plant .This gene was named BrUBC11 ( GenBank accession No .KM396887 ) .BrUBC1 I was 685 bp in full length cDNA and 447 bp in full length open reading frame ( ORF) encoding 148 amino acids .BrUBC11-GFP was localized to nucleus ,indicating that BrUBC 11 may play an important role in the nucleus .Quantitative-PCR analysis showed that the BrUBC11 was the highest expressed in the petal and less in bud .The expression of BrUBC11 in Tsuda exhibited tissue specificity .The expression of the BrUBC11 was induced by UV-A light in the swollen hypo-cotyls.These results indicated of that BrUBC11 had the potential to play a role in response to floral development and UV-A signal transduction pathways ,was a multifunction gene .%为阐释津田芜菁 BrUBC11基因的表达特性，克隆了津田芜菁 UBC11基因的全长 cDNA 序列，命名为BrUBC11，GenBank登录号为KM396887。其cDNA全长685 bp，ORF区全长447 bp，编码148个氨基酸的开放阅读框；亚细胞定位结果显示， BrUBC11-GFP定位于细胞核内，表明BrUBC11蛋白可能在细胞核中发挥其功能。荧光定量PCR检测BrUBC11在芜菁不同组织中的表达结果表明，该基因在花瓣中表达量最高，花蕾中次之，具有组织特异性。而且BrUBC11在芜菁白色根皮中的表达受长波紫外线（ UV-A）诱导。研究结果显示，在芜菁中，UBC11属于多功能基因，具有潜在的参与花发育和UV-A信号转导相关途径的功能。