高温和盐胁迫下硅对辣椒 CaMADS-box基因表达的影响

摘要

In order to study the influence of silicon on the expression of pepper CaMADS-box gene under abiotic stress,such as the high temperature and salt stress,we used pepper 1 01 as experimental materials,the physical and chemical properties of encoding protein was analyzed,phylogenetic tree was constructed,subcellu-lar localization was predicted through bioinformatics software on the base of the cloning of pepper CaMADS-box gene.The results showed that the cloned gene encoding protein CaMADS-box was hydrophilic protein,contai-ning MADS domain structure,belonged to MADS gene families.And its subcellular localization was in the nucle-us,the molecular evolutionary tree showed that close to Nicotiana,the similarity was 67%.Fluorescence quantitative analysis showed that CaMADS-box gene expression after high temperature stress and salt stress were presented first rise after the fall of the model,the difference was to peak at 48 h,under high temperature stress,salt stress peak at 24 h,silicon handle could induce gene expression CaMADS-box,under high temperature stress and salt stress were expressed at 1 2 h to reach peak,which suggested that CaMADS-box was a silicon quick response genes,spec-ulated that the silicon handle in alleviating pepper abiotic stress such as high temperature and salt stress plays an important role.%为了探索非生物胁迫下硅对辣椒 CaMADS-box 基因表达的影响，以豫椒101为材料，在对辣椒 CaMADS-box基因片段同源克隆的基础上，对其编码的部分蛋白进行氨基酸同源性分析、理化性质预测、亚细胞定位预测和进化树分析，探讨了硅处理后辣椒 CaMADS-box 基因在高温胁迫、盐胁迫下表达量的变化，结果表明，克隆得到的 CaMADS-box 基因所编码的蛋白为亲水性蛋白，含有 MADS 结构域，属于 MADS 基因家族，亚细胞定位在细胞核中，分子进化树表明其与茄属和烟草属亲缘关系最近，相似性达到67％。荧光定量分析表明，CaMADS-box 基因在高温胁迫和盐胁迫后表达量都呈现出先升高后下降的模式，高温胁迫下48 h 达到峰值，而盐胁迫在24 h 达到峰值，硅处理能够诱导CaMADS-box基因表达，在高温胁迫和盐胁迫下都在12 h 达到高峰值，这表明 CaMADS-box 是一个硅快速响应基因，推测硅处理在缓解辣椒高温胁迫和盐胁迫等非生物胁迫方面具有重要作用。