陆地棉 EPSPS 基因全基因组分析

摘要

当前在 NCBI 中提交的来源于陆地棉的 EPSPS 基因有2个，随着陆地棉基因组测序结果的公布，为在陆地棉基因组中全面鉴定 EPSPS 基因的存在提供了便利条件。从陆地棉异源四倍体标准系 TM－1基因组数据库中共搜寻获得4个 EPSPS 同源基因，这4个基因分别分布在 A07、A12、D07和 D12亚基因组。从这4个基因的核苷酸序列、氨基酸序列和基因结构的比对，构建进化树的系统发育分析以及基因的转录情况研究来看，定位在 A07和 D07亚基因组的2个基因属于共线性高度同源基因，定位在 A12和 D12亚基因组的2个基因也是相同的情况。序列比对结果表明，已经在 NCBI 中提交的2个 EPSPS 基因分别是定位在 A12和 D12亚基因组上的基因，研究结果为定位在 A07和D07亚基因组上 EPSPS 基因的克隆和功能研究提供了基础理论依据。%Currently,two EPSPS genes from Gossypium hirsutum have been submitted to NCBI.It is more con-vinent to identify EPSPS genes in genome level of upland cotton comprehensively following the publication of Gos-sypium hirsutum genomic sequence.Four EPSPS homologous genes were identified from the genome sequence data-base of allotetraploid cotton (Gossypium hirsutum L.acc.TM-1),and they were found to be distributed on subge-nomes of A07,A12,D07 and D12.Under the comperation of nucleotide sequences,amino acid sequences and gene structures,phylogenetic analysis of constructing phylogenetic tree and the study of transcription situation of these four genes,it was more clearly that the two genes located on A07 and D07 subgenomes belong to highly homologous co-linear gene,and the two genes located on A12 and D12 subgenomes as well.Sequence alignment results showed that the two EPSPS genes submitted to NCBI were the two genes located on A12 and D12 subgenomes respectively. The study results of this paper would provide an theoretical basis on cloning and functional research of EPSPS genes which located on A07 and D07 subgenomes.