毒蛋白 A 链基因被共抑制的蓖麻种子中毒蛋白含量的测定

摘要

为了获得毒蛋白含量低的蓖麻转基因新材料，以毒蛋白 A 链基因被共抑制的转基因蓖麻植株的 T0、T1、T2种子为研究对象，对种子中的毒蛋白含量进行测定。结果表明，采用磷酸盐提取法、BCA 试剂盒、UVwin5紫外软件V5．1．0、BandScan 蛋白定量分析软件相结合的方法，测定种子中毒蛋白含量，获得了2个共抑制后稳定遗传的材料，即 T2－9－1、T2－15，毒蛋白 A 链含量均为0，毒蛋白 B 链含量分别为对照通蓖5号的77．63％，83．38％。这2个材料可以作为低毒蛋白含量的蓖麻材料进行推广。%The purpose of this experiment was to a chain gene toxic protein was co-suppressed in transgenic castor plants T0 ,T1 ,T2 seeds were determination of toxic protein content,obtain a low content of toxic protein ricin new transgenic material to lay the foundation.The results were as follows:we used phosphate extraction method,BCA kit method,UVwin5,ultravioletV5.1.0,BandScan protein quantitative analysis.,after obtained the two co-suppression stable genetic material,nameed T2-9-1,T2-15,A chain toxin protein content were 0.Contrast Tongliao 5 ricin B chain respectively 77.63%,83.38%,both materials could be used as low-toxic protein ricin material to promote.