利用InDel指纹图谱评价鲁西南地区簇生朝天椒品种的相似度

摘要

通过研究鲁西南地区簇生朝天椒生产用种的农艺性状,构建分子指纹图谱,为在分子水平结合农艺性状评价簇生朝天椒品种间的相似度提供支撑,同时也为簇生朝天椒的品种评价、品种保护及种子市场规范化管理奠定基础.以商品名不同的8个鲁西南地区簇生朝天椒品种为试验材料,进行单株簇数、每簇果数、鲜单果重、干单果重、色价等指标的调查,采用UPGMA聚类分析进行品种间的农艺性状相似度评价.采用试剂盒法提取基因组DNA,利用其他生物学性状差异较大的8份辣椒材料,筛选出25对多态性InDel引物,构建数字化InDel分子指纹图谱.利用农艺性状和DNA指纹图谱综合对簇生朝天椒进行品种相似度评价.通过农艺性状调查结果表明,8个商品名不同的簇生朝天椒品种抗倒伏性均较好、株型紧凑、产量较高、综合表现较一致;8个品种间遗传距离为0.78时聚为1个大类,各品种表型性状与遗传背景表现一致,亲缘关系相对较近,品种相似度较高.利用25对具有多态性的InDel引物进行8个品种的指纹图谱构建,共扩增出160个位点,扩增片段大小在130~350 bp,8个品种间只在引物CIDH267产生位点差异,扩增片段大小在180~200 bp.参照DNA指纹方法判定标准(NY/T1433-2007),品种SYJ-2和SYJ-6与品种SYJ-1、SYJ-3、SYJ-4、SYJ-5、SYJ-7、SYJ-8为疑似同种.基于农艺性状和25对InDel标记构建的DNA指纹图谱可有效评价品种间的相似度,8个商品名不同的簇生朝天椒品种间农艺性状差异较小、遗传背景较窄、相似度极高,同质化严重,且存在同物异名现象.%The agronomic traits of clustered pepper were studied in Southwest Shandong Province Area,molecu-lar fingerprinting were established,in order to support the similarity evaluation methods with molecular level and ag-ronomic traits,also to lay the foundation of varieties evaluation,varieties protection and varieties management.Eight clustered pepper varieties with different brand names were used as experimental materials,the number of single plants,fruit number per cluster,fresh weight per fruit,dry weight per fruit and color value were tested.UPGMA clus-ter analysis was used to evaluate the agronomic traits similarity among eight varieties.The genomic DNA was extrac-ted by the kit method. Eight pepper materials with different biological characters were used to select 25 polymorphic InDel primers,and establish InDel molecular fingerprinting. Using agronomic traits and DNA fingerprinting,similar-ity evaluation was carried out among eight clustered pepper varieties. Accorded to the results of agronomic traits,the eight varieties all had the characteristics of better lodging resistance,compact plant type,high yield,and consistent performance. When the genetic distance was 0.78,the eight varieties were clustered into one major category in the agronomic characters.The fingerprinting established with 25 pairs polymorphism InDel primers,160 sites were am-plified,and the amplified fragment size was 130-350 bp. In the InDel fingerprinting,there was only different in primers CIDH267 among 8 varieties,the amplified fragment size was 180-200 bp. Reference DNA fingerprinting method (NY/t1433-2007),SYJ-2,SYJ-6 and varieties SYJ-1,SYJ-3,SYJ-4,SYJ-5,SYJ-7,SYJ-8 were similar vari-eties.Based on agronomic traits and DNA fingerprinting could effectively evaluate the similarity among varieties. There were small agronomic characters differences,low level genetic diversity,higher similarity among the eight clustered pepper. So,the homogeneity and synonyms of clustered pepper was serious in this area.