从青藏高原牦牛粪中分离出1株可降解毒死蜱的细菌,经形态和16S rDNA序列分析,鉴定其为鞘氨醇单胞菌属(Sphingomonas sp.)细菌.对其进行重离子诱变并筛选出菌株Sph-25-T1,该菌株对100 mg·L-1毒死蜱的72 h降解率达98.44％,比出发菌株提高27.37％.对Sph-25-T1进行传代培养,第5代时其对毒死蜱的降解率依然保持在90％以上.以喷洒毒死蜱1周后的不同土壤浸出液或蔬菜叶片做培养基时,Sph-25-T1对毒死蜱的降解率达到29.68％～78.40％.%A strain with the ability of chlorpyrifos degradation was isolated from the yak dung on Qinghai-Tibet Plateau, and identified as Sphingomonas sp. Based on the phenotype and 16S Rdna sequence analysis. In present study, we used heavy-ion beam irradiation to mutagenize the strain and screened a mutant named Sph-25-Tl. The experiment results showed that the strain Sph-25-Tl strongly degraded chlorpyrifos added in the medium, the degradation rate of 100 mg ? L ~] chlorpyrifos added in medium was by 98. 44% after 72h and 27. 37% higher than that of the original strain. The strain Sph-25-Tl also degraded chlorpyrifos by 90% above after five generations cultivation. In the mediums of soil extract and vegetable leaves after spraying chlorpyrifos for one week, the strain Sph-25-Tl had the degradation rate of chlorpyrifos by 29. 68% -78. 40% after 84 h.
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