基于线粒体控制区全序列的鹿亚科系统发育分析

摘要

The mitochondrial DNA(mtDNA) complete control region(D-loop) of 13 species of Cervinae was determined by direct DNA sequencing in order to study the taxonomy and phylogeny of the Cervinae. In addition the homologous sequences of 12 species of Cervinae were obtained from the GenBank. Total of 25 mt DNA D-loop of Cervinae were analyzed. The results showed that the length of D-loop is 914-1 072 bp. DNA difference ranged from 0. 1% -12. 2%, and divergence were 8. 0% -12. 2% among each of four genera. The molecular phylogenetic tree was contructed based on their D-loop sequences. The results indicated that red deer divided into two distinct groups, and Elaphurus, Hog deer and fallow deer should be incorporated into cervus, Eld's deer was the most antique one among cervus.%测定13种鹿亚科动物线粒体DNA控制区全序列,并结合从GenBank获得的12种鹿亚科动物同源序列进行分析,进一步研究鹿亚科物种的分类和系统进化.结果显示,25种鹿亚科动物线粒体控制区序列全长为914～1 072 bp,个体间序列差异为0.1％～12.2％,4个属间差异为8.0％～12.2％.构建的系统发育树结果表明,马鹿分为2个不同类群,麋鹿属的麋鹿、斑鹿属的豚鹿以及黇鹿属的黇鹿与鹿属的分化处于属间差异,支持将其并入鹿属的观点,坡鹿为鹿属中最原始的种.