TP-M13-SSR技术在枸杞遗传多样性研究中的应用

摘要

An economical detection method for simple sequence repeat with tailed primer M13 (TP-M13-SSR) was used in genetic diversity analysis for 29 wolfberry germplasm.Thirteen pairs of SSR primers screened amplified 78 alleles with an average of six alleles per locus.The average of MAF,NE,Ho,HE,I and PIC was 0.625,2.684,0.401,0.514,1.103 and 0.487,respectively.The similarity coefficients among 29 wolfberry germplasm ranged from 0.66 to 0.97,with average of 0.81.29 wolfberry germplasm could be divide into five groups and seven subgroups.The results showed there was a low level of genetic diversity among 29 wolfberry germplasm.The method had the advantages of economy,sensitiveness and high efficiency,and it had been used in genetic diversity analysis of wolfberry successfully.%利用在SSR扩增产物检测过程中的一种基于荧光测序技术的高通量低成本分析技术体系TP-M13-SSR对29份枸杞种质资源进行遗传多样性研究.13对引物共检测到78个等位基因,平均每个位点6个等位基因.群体平均的主等位基因频率(MAF)、有效等位基因数(NE)、观测杂合度(HO)、期望杂合度(HE)、Shannon's信息指数(I)和多态信息含量(PIC)分别为0.625、2.684、0.401、0.514、1.103和0.487.聚类分析表明:29份枸杞种质间相似系数为0.66～0.97,平均相似系数为0.81;供试材料可分为5大类7亚类.研究表明,供试材料的遗传相似性较高,遗传多样性较低.TP-M13-SSR方法具有经济、灵敏、高效等优点,在枸杞遗传多样性研究中应用效果好.