人羊水干细胞的分离培养及鉴定

摘要

目的：分离培养人羊水干细胞（ hAFSC ）并鉴定。方法：使用贴壁法分离培养羊水干细胞，细胞免疫荧光法和Western blot鉴定hAFSC。结果：分离的羊水干细胞均表达特异性标记物Oct-4、c-kit、SSEA-4、CD105。结论：成功分离及培养hAFSC，可作为种子细胞用于干细胞移植治疗肝纤维化。%Objective:To isolate,culture and identify the stem cells de-rived form human amniotic fluid(hAFSC).Methods:hAFSCs were isola-ted and cultured with adherent method ,and identified by cell immune flu-orescence and Western blotting.Results:Generally,the isolated amniotic stem cells expressed specific markers of Oct-4, c-kit, SSEA-4, and CD105.Conclusion:hAFSC is successfully isolated and cultured,which can be used as the seed cells for management of hepatic fibrosis through stem cell transplantation.