大鼠全脑缺血再灌注损伤富氢液不同时间点给药的疗效比较

摘要

目的：评价再灌注后富氢液不同时间点给药，对大鼠全脑缺血再灌注损伤的影响。方法：清洁级成年雄性SD大鼠72只，随机均分为4组：假手术组（S组）、全脑缺血再灌注损伤组（I／R组）、再灌注即刻给药组（H1组）、再灌注期间给药组（H2组）。 I／R组、H1组、H2组按照四血管阻塞法制作全脑缺血再灌注损伤模型。 H1组大鼠在再灌注即刻，经腹腔注射富氢液5 mL／kg（浓度0．6 mmol／L）。 H2组大鼠在再灌注后6 h，经腹腔注射富氢液5 mL／kg（浓度0．6 mmol／L）。 I／R组大鼠经腹腔注射等容量的生理盐水。再灌注24 h后，分别测定海马组织丙二醛（MDA）、肿瘤坏死因子－α（TNF－α）和白细胞介素－6（IL－6）的含量。结果：与I／R组相比较，H1组和H2组海马组织的MDA含量、TNF－α、IL－6水平降低（P＜0．05）。 H1组与H2组海马组织的MDA含量、TNF－α和IL－6水平无显著差异（ P＞0．05）。结论：再灌注后不同时间点给予富氢液均能改善大鼠全脑缺血再灌注损伤，但两种给药方法之间无明显差异。%Objective:To observe the effect of hydrogen-rich saline given at different time point after reperfusion in rats with transient global cerebral is-chemia-reperfusion.Methods:Adult male Sprague-Dawley rats of clean grade were randomized into 4 groups: i.e., sham operative group (S), cerebral ischemia-reperfusion group ( I/R) , I/R plus hydrogen-rich saline injected at the beginning of reperfusion group ( H1 ) , and I/R plus hydrogen-rich saline injected at 6h after reperfusion group (H2).Rat models of global cerebral ischemia-reperfusion were developed with four-vessel occlusion technique for groups of I/R, H1 and H2 .Rats in H1 group were intraperitoneally injected with hydrogen-rich saline in dose of 5mL/kg(0.6 mmol/L) immediately after reperfusion, and those in H2 group were intraperitoneally administered with the same agent and same dosage after 6 h of reperfusion.Rats in I/R group were given equal volume of normal saline.At 24 h of reperfusion, the hippocampus was taken from the above animals and detected for malondialdehyde (MDA), tumor necrosis factor-α(TNF-α) and interlukin-6 (IL-6) level.Results:Compared to I/R group, the concentrations of MDA, TNF-αand IL-6 were significantly reduced in H1 and H2 group (P <0.05), yet the difference was not significant between H1 group and H2 group (P >0.05). Conclusion:Intraperitoneal administration of hydrogen-rich saline at different time point after reperfusion may reduce the brain injury of rats suffered from global cerebral ischemia-reperfusion.However, there is no difference in the efficacy between the two administration methods .