亚急性酒精中毒戒断对大鼠痛觉时反应量-效关系的影响

摘要

目的:观察亚急性酒精中毒戒断对大鼠光辐射热甩尾反射时反应量-效关系(TDRR)的影响.方法:取雄性SD大鼠随机分为生理盐水组(NS,n=9)和亚急性酒精中毒组(SA,n=6).SA组大鼠给予56%乙醇灌胃10 d(15 mL/kg,qd)造模,NS组大鼠给予等容量的生理盐水.停止酒精灌胃后6h观察戒断症状,并在戒断1d、3d、7d、10d、14d,应用移动光辐射热甩尾反射法重复检测大鼠光辐射热甩尾反射的潜伏期(TFL)和光照强度(Focus值)之间的TRDD.结果:①与NS组相比,SA组的体质量增长在造模期间无明显差异,但戒断期间明显受抑(P<0.05).②SA组在戒断1 d时Focus值为23的TFL比NS组缩短(P<0.05),且2组大鼠戒断后TFL的TDRR重复测量双因素方差分析显示,光照强度因素、组间因素以及光照强度因素与组间因素的交互作用均有统计学意义(P<0.01).③对戒断1 d和14 d的2组TDRR进行双曲线型四参数模型Y=cs+1/(x-a)s+b的非线性拟合分析表明,SA组在戒断1 d的参数c减小,3~14 d逐渐恢复.结论:亚急性酒精中毒戒断第1天出现痛觉敏化,随后逐渐恢复.%Objective:To observe the withdrawal effects of subacute alcoholism on timed dose-response relationship ( TDRR) for tail-flick response in-duced by light radiant heat in rats.Methods:Fifteen male SD rats were randomized into normal saline administration group (NS,n=9) and subacute alco-holism group (SA,n=6).Rats in SA group were intragastrically given 56%alcohol solution (15 mL/kg)(qd) for 10 days,and those in NS group were administrated by saline with corresponding content.Withdrawal symptoms were observed in rats at 6h after cessation.The tail-flick latency (TFL) evoked by diverse light intensity ( Focus=10,15,23,34,51,76) was measured with light beam analgesy-meter at moved points of tail on the 1st,3rd,7th,10th and 14th day after withdrawal,and then the TDRR was analyzed between the light intensity and TFL .Results:①Body weight increase remained no notable difference between rats in NS and SA group,yet rats in SA group were less increased after withdrawal (P<0.05);②TFLs at Focus=23 and 1-day with-drawal were shorter for rats in SA group than NS group (P<0.05).Two-way ANOVA analysis of TFL data for rats in two groups showed that factors of light-intensity and group, as well as interaction between group and light-intensity were all significant ( P<0.01);③The TDRR data of TFLs at 1-day and 14-day withdrawal for rats in 2 groups were nonlinearly fitted to the hyperbolic 4-parameter model Y=cs+1/(x-a)s+b,and the parameters showed that pa-rameter c in TDRR of 1-day withdrawal in SA group was decreased and then recovered during 3-to 14-day withdrawal.Conclusion:The results suggest that withdrawal of subacute alcoholism may dramatically induce pain sensitization in rats ,and the pain response tends to recover in 3 to 14 days following with-drawal.