燃煤型氟中毒对大鼠睾丸组织氧化应激效应的影响

摘要

Objective To explore the effect of endemic fluoride poisoning caused by coal burning on the oxidative stress in rat testis. Methods Totally 40 male SD rats were equally randomized into four groups:control group, low fluororsis group, middle fluororsis group, and high fluorosis group. Rats in all three fluoror-sis groups were fed with corn dried by burning coal obtained from endemic fluorosis areas with high fluoride, and thus the animal models of fluorosis were established. After 120 and 180 days, all the rats were sacrificed. Testis tissues were stained with hematoxylin-eosin and observed under light microscope. The malonaldehyde ( MDA) content, superoxide dismutase (SOD) activity, total nitric oxide synthase ( T-NOS) , and iducible nitric oxidase synthase (iNOS) were measured by biochemical methods in the testis tissues. The content of NaF in testis was measured by fluorine selective electrode. Results The rat fluororsis models were successfully established. The fluoride content in testis was significantly increased in all the fluororsis groups (P < 0. 01). Testicular structures were damaged in all of fluoride groups. The T-NOS, iNOS activities, and MDA content of each fluoride group were significantly higher than that of the control group on day 120 and 180 (P<0. 05 or 0. 01). The T-NOS, iNOS activities, and MDA content significantly increased in a dose-dependent manner (P < 0. 05 or 0. 01). The SOD activities significantly decreased in all the fluoride groups ( P < 0. 05 or 0. 01 ) . Conclusions Endemic fluoride poisoning caused by coal burning can cause disorders in the oxidative system and anti-oxidative system in rat testis. The oxidative stress may play an important role in the fluorides-induced reproductive toxicity in male rats.%目的 研究燃煤型氟中毒对大鼠睾丸组织氧化效应的影响,探讨燃煤型氟中毒的生殖毒害机制.方法 将40只雄性SD大鼠随机分为对照组、低氟组、中氟组、高氟组4组,每组10只.各染氟组喂饲含不同比例的燃煤型氟中毒病区煤烘玉米的饲料,构建燃煤型氟中毒动物模型.分别于120、180 d分批处死,各时间点每组处死大鼠数为5只.采用HE染色,光镜下观察睾丸组织病理学改变；制备睾丸组织匀浆,检测睾丸组织中丙二醛(MDA)含量及超氧化物歧化酶( SOD)、总一氧化氮合酶(T-NOS)和诱导型一氧化氮合酶(iNOS)活性；氟离子选择电极法检测大鼠尿氟含量及睾丸组织氟含量.结果 成功建立大鼠氟中毒模型.各染氟组大鼠睾丸组织氟含量均明显升高(P均＜0.01),睾丸生精小管均发生不同程度的损害.120d和180 d时,各染氟组大鼠睾丸组织T-NOS、iNOS活性和MDA含量均明显升高(P＜0.05或P＜0.01),且随染氟剂量增加而升高；120d和180 d时,各染氟组大鼠睾丸SOD活性较对照组明显降低(P＜0.05或P＜0.01).结论 燃煤型氟中毒可导致大鼠睾丸内氧化系统与抗氧化系统失衡,生殖细胞受损.氧化应激损伤在燃煤型氟中毒所致的雄性大鼠生殖毒性中可能发挥重要作用.