三氯醋酸-丙酮沉淀法用于骨组织蛋白的提取

摘要

Objective To explore the feasibility and efficiency of extracting protein from bone tissue using trichloroacetic acid (TCA) -acetone precipitation method.Methods Hydrochloric acid (HCL) decalcification method and TCA-acetone precipitation method were separately used for bone protein extraction.The efficiencies of these two methods were compared.Results TCA-acetone precipitation method had significantly higher extraction efficiency.Compared with HCL decalcification method, it had less pollution from bone marrow hematopoietic tissue.Protein band distribution was similar between these two methods.Conclusion TCA-acetone precipitation method is useful for bone proteomics research.%目的 探讨三氯醋酸-丙酮沉淀法提取骨组织蛋白的可行性和效率.方法 采用盐酸脱钙法和三氯醋酸-丙酮沉淀法,分别用于骨组织蛋白的提取,并对两种方法的提取效率进行对比.结果 三氯醋酸-丙酮沉淀法可获得较高的骨蛋白提取率,与盐酸脱钙法相比,分子条带分布范围相近,且不受骨髓造血组织影响.结论 三氯醋酸-丙酮沉淀法可以用于骨组织蛋白质组的研究.