耐亚胺培南鲍曼不动杆菌苯唑西林基因型分析

摘要

Objective To provide the scientific evidence for the control of hospital infection with imipenem-resistant Acinetobacter baumannii and its epidemiological survey by investigating the prevalence of its OXA genotypes in our hospital. Methods The OXA genetypes of 68 imipenem-resistant Acinetobacter baumannii strains isolated in our hospital from April 2010 to December 2010 were analyzed by multiplex PCR. Results Of the 68 isolated imipenem-resistant Acinetobacter baumannii strains, 7 were OXA-51 positive gene with a detection rate of 10.29%, 60 were OXA-51 and OXA-23 positive gene with a detection rate of 88.24%, and 1 was OXA-51 and OXA-58 positive gene with a detection rate of 1.47%. No OXA-24 gene was detected. Conclusion OXA-51+OXA-23 genotype is the popular OXA genetype of imipenem-resistant Acinetobacter baumannii in our hospital, indicating that it is the most important mechanism underlying the resistance of imipenem-resistant Acinetobacter baumannii to carbapenemases in our hospital, and laboratory should strengthen its monitoring of enzyme-producing strains.%　　目的了解我院耐亚胺培南鲍曼不动杆菌携带苯唑西林(oxacillin,OXA)基因型的情况,为医院感染监控及流行病学调查提供科学依据.方法采用多重PCR方法,分析2010年4-12月我院住院患者分离的耐亚胺培南鲍曼不动杆菌68株的OXA基因型.结果68株耐亚胺培南鲍曼不动杆菌中,7株只携带OXA-51基因(检出率约为10.29%),60株同时携带OXA-51和OXA-23基因(检出率约为88.24%),1株同时携带OXA-51和OXA-58基因(检出率约为1.47%),未扩增到OXA-24基因.结论我院耐亚胺培南鲍曼不动杆菌携带OXA基因以OXA-51+OXA-23基因型为主,实验室应加强对产酶菌株的监测.