Molecular detection and drought resistance analysis of SacB-transgenic poplars (Populus alba ×P.glandulosa)

摘要

The propagation of drought-resistant transgenic poplars is one of the more effective ways to improve the ecological state of arid regions.The expression of SacB gene in transgenic lines of Populus alba × P.glandulosa was analyzed by a semi-quantitative RT-PCR.Drought resistance of these transgenic lines was evaluated by water stress trials in a greenhouse.Results showed that the mRNA transcription of SacB was found in all transgenic lines and fructan has accumulated in the leaves of these plants.The growth,biomass and leaf water content of some transgenic lines were significantly higher than those of the control plants under drought stress.Correlation analysis indicated that the said factors were significantly and positively related to the concentration of fructan in the leaves of the transgenic lines.Results revealed that the expression of SacB gene in transgenic poplars enhanced its resistance to water stress.