首页> 外文学位 >Mobile phospholipid bilayers on a polyion/alkylthiol layer pair as a biosensor model for selective peptide detection.
【24h】

Mobile phospholipid bilayers on a polyion/alkylthiol layer pair as a biosensor model for selective peptide detection.

机译:聚离子/烷硫醇层对上的可移动磷脂双层对作为用于选择性肽段检测的生物传感器模型。

获取原文
获取原文并翻译 | 示例

摘要

Solid supported phospholipid bilayers, as model biomembranes, draw great attraction in biosensor applications as well as the biophysics and biochemistry fields. To mimic the natural cell membranes, it becomes very important to minimize the effects of the close proximity of solid substrates, which may hinder the lateral diffusivity of the model membranes and limit the space for transmembrane protrusions of proteins and peptides. This dissertation describes the use of a polyelectrolyte/alkylthiol layer pair that lifts the model membranes away from the solid substrates, creating aqueous environments on both sides of the membranes and retaining the mobility of the membranes. Lipid mixtures of major components of natural cell membranes, phosphatidylcholine (PC), phosphatidylserine (PS) and cholesterol, are used to build bilayers on the polyion/alkylthiol layer pair by electrostatic attractions. The kinetics of the bilayer formation and the thicknesses of the bilayers are examined by surface plasmon resonance (SPR). The lateral mobility of the bilayers is studied by fluorescence recovery after photobleaching (FRAP).; A second major part of this dissertation revolves around the insertion of pore forming peptides and the insulating properties of the supported membranes, which are important for biosensor fabrications. If the supported membranes have enough structural integrity and thus charge blockage, the effect of the ion-channels formed by the inserted peptides can be easily detected due to facilitated charge transfer. Electrochemical experiments by cyclic voltammetry (CV) are performed with SPR in a combined set-up to study the insulating properties of the supported bilayers with different lipid compositions. Ion-channel forming peptides and antimicrobial peptides are introduced to the supported membranes. The association of the peptides with the membranes is detected by the SPR and the channel-forming activities of the peptides are measured by the CV experiments in an electrochemical cell.; The third part of the dissertation focused on the use of atomic force microscopy (AFM) to monitor the surface morphology change with the layer-by-layer deposition in situ. The adsorption process is presented in correlation to the electrochemical (CV) and SPR studies in the second part of the dissertation. The soft-contact mode used in this study is able to detect the nanoscopic architecture of the biomembranes and the effect of the peptides associated with the membranes.
机译:固体负载的磷脂双层作为模型生物膜,在生物传感器应用以及生物物理学和生物化学领域吸引了极大的吸引力。为了模拟天然细胞膜,将固体底物的紧密结合的影响降至最低非常重要,这可能会阻碍模型膜的横向扩散,并限制蛋白质和多肽跨膜突出的空间。本论文描述了聚电解质/烷基硫醇层对的使用,该对对将模型膜提离固体底物,在膜的两侧形成水性环境并保持膜的迁移率。天然细胞膜主要成分,磷脂酰胆碱(PC),磷脂酰丝氨酸(PS)和胆固醇的脂质混合物可通过静电吸引作用在聚离子/烷基硫醇层对上形成双层。双层形成的动力学和双层的厚度通过表面等离子体激元共振(SPR)检查。通过光漂白后的荧光恢复(FRAP)研究了双层的横向迁移率。本论文的第二个主要部分围绕成孔肽的插入和被支撑膜的绝缘特性,这对生物传感器的制造很重要。如果所支持的膜具有足够的结构完整性并因此阻止了电荷,则由于促进了电荷转移,可以容易地检测到由插入的肽形成的离子通道的作用。在组合设置中,使用SPR进行了通过循环伏安法(CV)进行的电化学实验,以研究具有不同脂质组成的双层支撑膜的绝缘性能。将离子通道形成肽和抗菌肽引入到支持的膜中。通过SPR检测肽与膜的缔合,并通过电化学电池中的CV实验测量肽的通道形成活性。论文的第三部分着重于利用原子力显微镜(AFM)监测表面形貌随原位逐层沉积的变化。论文的第二部分介绍了与电化学(CV)和SPR研究相关的吸附过程。本研究中使用的软接触模式能够检测生物膜的纳米结构以及与膜相关的肽的作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号