Molecular characterization of citrus partial hybrids produced by microprotoplast mediated chromosome transfer.

摘要

Citrus is an important commercial fruit crop in the world. There is a lot of genetic variability within citrus and related genera but the scope of development of improved cultivars in citrus by sexual hybridization is limited due to several reproductive barriers such as nucellar polyembryony, apomixis, pollen and ovule sterility; and gametophytic self- and cross-incompatibility which prevent potentially useful hybridizations from being successful. Advances in cell and molecular biology offer the possibility of creating new gene combinations by methods such as somatic hybridization via protoplast fusion, genetic transformation and microprotoplast mediated chromosome transfer.; Many of the citrus species have long juvenile phases, and large tree size, making hybrid evaluation a long process and a costly endeavor. Molecular methods such as Random Amplified Polymorphic DNA, Amplified Fragment Length Polymorphism, Restriction Fragment Length Polymorphism, and several others marker assisted assays have been reported in citrus which facilitated creation of linkage maps, genotype characterization, screening of cultivars and hybrids. In this research project, two sets of partial hybrids namely 'Seleta' sweet orange (C. sinensis L. Osbeck) + 'Red Marsh' grapefruit (C. paradisi Macf.) herein referred as SR hybrids and 'Murcott' tangor (C. reticulata x C. sinensis) + 'ChangSha' mandarin (C. reticulata ) hybrids here referred to as MC hybrids; which were created by MMCT procedures, were molecular characterized in comparison with the recipient and donor parents. (Abstract shortened by UMI.)