声明
Chapter 1 Literature Review
Introduction
1.1 High Performance Liquid Chromatography
1.1.1 Reversed-Phase Chromatography
1.1.2 Ion-Pair Chromatography
1.1.3 Mixed-Mode Chromatography
1.2 Synthesis of Stationary Phases
1.2.1 Reaction Methods
1.2.2 Reaction Conditions
1.3 Retention Mechanisms
1.3.1 Reversed-Phase Mode
1.3.2 Ion Exchange Mode
1.3.3 Mixed-Mode
1.4 Fixed-Dose Combination Drugs
1.4.1 Introduction
1.4.2 Analysis of Current Situation
1.5 Goal and Objectives
Chapter 2 Experimental
2.1 Chemicals and Equipment
2.1.1 Chemicals
2.1.2 Equipment
2.2 Synthesis of Stationary Phases
2.2.1 Epoxide Modified Silica
2.2.2 Diol Modified Silica
2.2.3 Octadecyl Ester Modified Silica
2.2.4 Sulfonic Modified Silica
2.2.5 Octadecyl/Sulfonic Modified Silica
2.3 Characterization
2.4 Chromatographic Evaluation
2.4.1 Column Packing
2.4.2 Reversed-Phase Mode
2.4.3 Ion Exchange Interaction Mode
2.4.4 Hydrophilic Interaction Mode
2.4.5 Mixed-Interaction Mode
2.5 Influence Factors of Mixed-Mode Retention
2.5.1 Stationary Phases Modes
2.5.2 Effect of Mobile Phases Composition on Sulfonic Stationary Phase
2.5.3 Effect of Samples on Octadecyl/Sulfonic Stationary Phase
2.6 Applications
2.6.1 Compound Methoxyphenamine Hydrochloride Capsules
2.6.2 Compound Dihydralazine Sulfate Tablets
2.6.3 Compound Reserpine/Triamterene Tablets
2.6.4 Compound Reserpine Tablets
Chapter 3 Synthesis and Characterization of Mixed-Mode Stationary Phases
Introduction
3.1 Synthesis
3.2 Characterization
3.2.1 Fourier Transform Infrared Spectroscopy (FTIR)
3.2.2 13C Solid State Nuclear Magnetic Resonance Spectra(13C NMR)
3.2.3 Elemental Analysis
3.3 Chromatographic Evaluation
3.3.1 Reversed-Phase Mode
3.3.2 Ion Exchange Interaction Mode
3.3.3 Hydrophilic Interaction Mode
3.3.4 Mixed-Interaction Mode
3.4 Summary
Chapter 4 Influence Factors of Mixed-Mode Retention
Introduction
4.1 Stationary Phases Modes
4.1.1 Salt Concentration
4.1.2 Organic Concentration
4.2 Effect of Mobile Phases Composition on Sulfonic Stationary Phase
4.2.1 Alkylaniline Homologues
4.2.2 Basic Drugs
4.3 Effect of Samples on Octadecyl/Sulfonic Stationary Phase
4.3.1 Different Structures Homologues
4.3.2 Basic Drugs
4.4 Summary
Chapter 5 Applications
Introduction
5.1 Compound Methoxyphenamine Hydrochloride Capsules
5.2 Compound Dihydralazine Sulfate Tablets
5.3 Compound Reserpine/Triamterene Tablets
5.4 Compound Reserpine Tablets
5.5 Summary
Chapter 6 Conclusions and Outlooks
6.1 Conclusions
6.2 Outlooks
参考文献
Appendices
Notes on Publications and Participation in Scientific Research
致谢
天津大学;
