声明
ABBREVIATIONS
CHAPTER 1 Background
1.1 Mammalian hibernation
1.2 The changes in mammalian skeletal muscle during hibernation
1.3 The MicroRNAs and its functions
1.3.1 The discovery of miRNAs
1.3.2 Introduction to miRNAs in hibernation
1.4 The PI3K/AKT/mTOR pathway and its functions
1.5 Study progress of miRNAs on PI3K/AKT/mTOR pathway in skeletal muscle
1.6 Purpose and significance of present study
CHAPTER 2 The changes of body mass and muscle mass of Daurian ground squirrels
2.1 Daurian ground squirrels in present study
2.2 The monitoring and grouping of Daurian ground squirrels
2.2.1 Body temperature monitoring
2.2.2 Experimental animal grouping
2.3 Skeletal muscle sample collection
2.3.1 Selection of semitendinosus muscle for present study
2.3.2 Animal anesthesia
2.3.3 Isolation of ST muscle
2.3.4 The changes of body mass of Daurian ground squirrels
2.3.5 The changes of ST muscle mass
2.4 Brief summary
CHAPTER 3 The expression levels of four miRNAs in ST muscle
3.1 Selection of miRNAs
3.1.1 miR-206
3.1.2 miR-29b
3.1.3 miR-21a
3.1.4 miR-99b
3.2 Experimental methods
3.2.1 Identifying the miRNAs sequence
3.2.2 Primer designing
3.2.3 Main instruments and reagents
3.2.4 Extraction of miRNAs from ST muscle
3.2.5 cDNA synthesis from miRNA
3.2.6 Real time-PCR detection of miRNAs
3.2.7 Data statistics and analysis
3.3 Experimental results
3.3.1 The expression
3.3.2 The expression levels
3.3.3 The expression
3.3.4 The expression
3.4 Brief summary
CHAPTER 4 The expression levels of PI3K/AKT/mTOR in ST muscle
4.1 The PI3K/AKT/mTOR signaling pathway
4.2 Experimental methods
4.2.1 Main instruments and reagents
4.2.2 The formulations of solutions
4.2.3 Extraction of protein from ST muscle
4.2.4 Western Blotting
4.2.5 Data statistics and analysis
4.3 Experimental results
4.3.1 The expression levels
4.3.2 The expression levels
4.3.3 The expression levels
4.4 Brief summary
CHAPTER 5 Discussion and conclusion
5.1 Discussion
5.2 Conclusion
参考文献
致谢
Research achievements during the study for the Master’s degree
西北大学;
