声明
Abbreviations
1 Introduction
1.1 Physiological features in seed germination
1.2 Transcriptomic approach to address seed germination
1.2.1 Energy production for germination
1.2.2 Genes involve in starch and sucrose metabolism
1.2.3 Genes involve in protein mobilization
1.2.4 Activation of developmental genes
1.2.5 Defense genes activation
1.2.6 Phytohormones in seed germination
1.3 Proteomic approach to address seed germination
1.3.1 Proteins involved in regulating germination
1.3.2 Progress in proteomics related to seed germination
1.3.3 Proteins involved in seed germination
1.4 Transcriptome and proteomics interaction
1.4.1 Transcription and translation activation
1.4.2 Translation role in seed germination
1.4.3 Comparison of the seed transcriptome and proteome
1.4.4 Post-translational control of seed germination
1.5 Objectives and technical route
1.5.1 Objectives
1.5.2 Technical route
2 Transcriptome analysis in Cyclobalnopsis gilva seed germination
2.1 Materials and methods
2.1.1 Plant materials
2.1.2 RNA extraction and construction of cDNA libraries for transcriptome sequencing
2.1.3 Illumina sequencing, assembly and annotation
2.1.4 Analysis of quantitative real-time PCR (qRT-PCR)
2.1.5 Analysis of physiological parameters
2.1.6 Statistical analysis
2.2 Results
2.2.1 Sequence analysis and assembly from C. gilva seeds
2.2.2 Analysis of differentially expressed unigenes (DEGs) by RNA-seq
2.2.3 DEGs validation during seed germination
2.2.4 Physiological profiles during seed germination
2.3 Discussion
2.3.1 Transcriptional responses in the glycolysis and TCA cycle pathways
2.3.2 Transcriptional responses in starch and sucrose metabolism
3 Proteomic study on Cyclobalnopsis gilva seed germination
3.1 Proteomic 2-DE approach to address low germination rate in C. gilva seeds
3.1.1 Materials and methods
3.1.2 Results
3.1.3 Discussion
3.2 Label-Free quantitative proteomics analysis in C. gilva seed germination
3.2.1 Materials and methods
3.2.2 Results
3.2.3 Discussion
4 Conclusion
参考文献
Publication
致谢
福建农林大学;
