声明
Chapter 1 Literature Review
1.1 Mesoporous Materials as Drug Delivery Systems
1.1.1 Metallic materials-based mesoporous systems
1.1.2 Nonmetallic materials-based mesoporous systems
1.1.3 Metal organic frameworks
1.2 Stimuli-Responsive Nanocarriers for Drug Delivery
1.2.1 Endogenous triggers
1.2.2 Exogenous triggers
1.3 Hypoxia Microenvironment in Solid Tumor and Its Application in Drug Delivery
1.3.1 Hypoxia microenvironment in solid tumors
1.3.2 Hypoxia-responsive moieties in drug delivery systems
1.4 Hypothesis and aim
Chapter 2 Preparation of Drug-Loaded Hypoxia-SensitiveMesoporous Silica Nanocarriers
2.1 Introduction
2.2 Materials and Instruments
2.2.1 Materials
2.2.2 Instruments
2.3 Preparation and Characterization of Mesoporous Silica Nanocarriers
2.3.1 Preparation of mesoporous silica nanocarriers
2.3.2 Characterization of mesoporous silica nanocarriers
2.3.3 Results
2.4 Preparation and Characterization of Hypoxia-Sensitive Polymer Coating (pDAB)
2.4.1 Preparation of MSN/pDAB and pDAB
2.4.2 Characterization of pDAB
2.4.3 Results
2.5 Preparation and Characterization of Drug-Loaded Hypoxia-Sensitive Coated Mesoporous Nanocarriers(Ce6@MSN/pDAB/F68)
2.5.1 Preparation of Ce6@MSN/pDAB/F68
2.5.2 Drug loading
2.5.3 Characterization of Ce6@MSN/pDAB/F68
2.5.4 Results
2.6 Discussion
Chapter 3 Degradation of pDAB under a Mimicked HypoxicEnvironment in vitro
3.1 Introduction
3.2 Materials and Instruments
3.2.1 Materials
3.2.2 Instruments
3.3 Verification of pDAB Reduction under Hypoxia
3.3.1 Preparation of sample solution
3.3.2 Spectroscopic analysis
3.3.3 Results
3.4 Detection of Polymer Degradation under Hypoxia
3.4.1 Introduction of 4-(dimethylamino)benzaldehyde
3.4.2 Preparation of chromogenic solution
3.4.3 Detection of degradation products
3.4.4 Results
3.5 Discussion
Chapter 4 Fluorescence Resonance Energy Transfer Analysis
4.1 Introduction
4.2 Materials and Instruments
4.2.1 Materials
4.2.2 Instruments
4.3 Preparation and Characterization of Cou6+RhoB@MSN/pDAB/F68
4.3.1 Preparation of Cou6+RhoB@MSN/pDAB/F68
4.3.2 Drug loading
4.3.3 Fluorescence spectrum of Cou6+RhoB@MSN/pDAB/F68
4.4 FRET Analysis in Sodium Dithionite Aqueous Medium
4.4.1 Stability of FRET probes in sodium dithionite solution
4.4.2 FRET analysis under mimicked hypoxia environment
4.5 FRET Analysis in Hypoxic Cells
4.5.1 FRET analysis in MCF-7 cells
4.5.2 Confocal Laser Scanning Microscope(CLSM) images Analysis
4.5.3 Results
4.6 FRET Analysis in vivo
4.6.1 FRET analysis in 4T1 tumor-bearing BALB/c mice
4.6.2 Results
4.7 Discussion
Chapter 5 Cytotoxicity Assay in MCF-7 Cells
5.1 Introduction
5.2.1 Materials
5.2.2 Instruments
5.3 Cytotoxicity Assay of Ce6@MSN/pDAB/F68
5.3.1 MTT assay of Ce6@MSN/pDAB/F68 in MCF-7 cells
5.3.2 Results
5.4 Cytotoxicity Assay of Dox@MSN/pDAB/F68
5.4.1 MTT assay of Dox@MSN/pDAB/F68 in MCF-7 cells
5.4.2 Results
5.5 Discussion
Chapter 6 Conclusion and Prospect
6.1 Conclusion
6.2 Prospect
参考文献
Publications
致谢
天津大学;
