声明
摘要
ABSTRACT
ABBREVIATIONS
CHAPTER 1 Literatu re ReView and Study Plan
2 Socio-economic importance of blunt snout bream farming
3 Fat Nutrition and HeaIth
4 Betaine
5 Choline
6．Choline and betaine metabolism
7 Lipid metaboIism and transportation
7．1 Lipid metaboIism
7．2 Lipid transportation
8 Fatty liver
9 Hypothesis and purpose of the study
CHAPTER 2 Effect of dietary betaine on growth performance，antioxidant capacity and lipid metabolism in blunt snout bream(Megalobrama amblycephala)fed a high-fat diet
Introduction
1 MateriaIs and methods
1．2 Fish and the feeding trial
1．3 Experimental design and diets
1．4 Sampnng and analysis
1．5 Growth performance
1．6 Proximate anaIysis
1．7 Analysis of liver antioxidant status
1．8 Determination of biochemical parameters
1．9 TotaI RNA extraction，reverse transcription and real-time PCR
2 Statistical analysis
3 Results
3．1 Effect of dietary supplementation on growth performance
3．2 WhoIe body composition
3．3 Hepatic anti-oxidant activities
3．4 Effects of betaine on Iipid metabolites
3．5 Effect of betaine on the expression of lipid metabolism-related genes
4 Discussion
5 Conclusion
CHAPTER 3 Effects of dietary betaine and choline association on lipid metabolism in blunt snout bream(Megalobrama amblycephala)fed a high-fat diet
Introduction
1 Materials and methods
1．1 Choline，betaine and Experimental diets
1．2 Experimental nsh and feeding triaI
1．3 Sampling and anaIysis
1．4 Growth performance
1．5 Proximate anaIysis
1．6 AnaIysis of liVer antioxidant status
1．7 Determination of biochemical parameters
1．8 Determination of the homocysteine level in the plasma
1．9 Determination of the SAM and SAH concentrations in the liver
2 Statistical analysis
3 Results
3．1 Effect of betaine and choline associated diets on growth performance
3．2 Whole body composition
3．3 Effect of dietary betaine and choline associated on antioxidant status
3．4 Effect of dietary betaine and choline association on biochemical parameters
3．5 Effect of Betaine and choIine associated on methylation capacity in the high-fat diet fed blunt snout bream
3．6 Relative transcriptions of genes related to lipid metabonsm
4 Discussion
5 Conclusion
Conclusion of the whole study
References
Acknowledgements
Acknowledgments
Meeting Attended and Publications
Academic award
南京农业大学;
