声明
LIST OF ABBREVIATIONS
CHAPTER INTRODUCTION
1.1 Whey Protein Overview
1.1.1 Chemical Composition
1.1.2 Major Whey Proteins
1.1.4 Functional Properties of Whey Proteins
1.2 Whey Protein-based Nanoparticles Encapsulation of Bioactive Compounds
1.2.1 Overview of Nanoparticles
1.2.2 Whey Protein-based Nanoparticles
1.2.3 Enhancement of Whey Proteins-based Nanoparticles for Bioactive Compounds by Thermal or Ultrasound Method
1.2.4 Advantages of Whey Protein-based Nanoparticles for Bioactive Compounds
1.3 3,3'-Diindolylmethane (DIM)
1.3.2 Role of 3,3′-Diindolylmethane in the Treatment of Castrate-resistant Prostate Cancer (CRPC) of Human Metastatic Prostate Cancer
1.4 Research Contents
1.4.1 Significance of the Research
1.4.2 Research Content
1.5 Innovation Points
1.6 Experimental Model
CHAPTER 2 Preparation and Characterization of Whey Protein Isolate-Dim Nanoparticles
2.1 Materials and Equipments
2.1.1 Materials and Reagents
2.1.2 Instruments and Equipments
2.2 Methodology
2.2.1 Nanoparticles Preparation
2.2.2 Determination of Particle size, Polydispersityindex(PDI) and Zeta Potential
2.2.3 Encapsulation Efficiency (EE)
2.2.4 Differential Scanning Calorimetry (DSC)
2.2.5 Determination of Rheological Properties
2.2.6 Fourier Transform Infrared Spectroscopy (FT-IR)
2.2.7 Transmission Electron Microscopy (TEM) Analysis
2.2.8 The Influence of Encapsulation on Stability of DIM
2.2.9 Experimental Design
2.3 Results and Discussion
2.3.1 Physicochemical Determination of DIM-encapsulated WPI Nanoparticles
2.3.2 Thermal Properties of the DIM-encapsulated WPI Nanoparticles
2.3.3 Rheological Properties of the DIM-encapsulated WPI Nanoparticles
2.3.4 Fourier Transform Infrared Spectroscopy (FT-IR)
2.3.5 Transmission Electron Microscopy (TEM)
2.3.6 Photochemical Stability
2.3.7 Impact of pH
2.4 Conclusions
CHAPTER 3 Physicochemical and Microstructural Properties of Polymerized Whey Protein Concentrate Encapsulated 3, 3'-Diindolylmethane Nanoparticles
3.1 Materials and Equipments
3.1.1 Materials and Reagents
3.1.2 Instruments and Equipments
3.2 Methodology
3.2.1 Sample Preparation
3.2.2 Particle Size and Zeta potential Measurement
3.2.3 Rheological Determinations
3.2.4 Fourier Transform Infrared (FTIR) Spectra Analysis
3.2.5 Transmission Electron Microscopy (TEM) Analysis
3.2.6 Storage Stability Analysis
3.2.7 Statistical Analysis
3.3 Results and Discussion
3.3.1 Particle Size and Zeta Potential of PWP–DIM Nanoparticles
3.3.2 Rheological Properties of PWP–DIM Nanoparticles
3.3.3 FTIR spectra of PWP–DIM Nanoparticles
3.3.4 Microstructure of PWP–DIM Nanoparticles
3.3.5 Changes in Color and Absorbance of PWP–DIM Nanoparticles During Storage
3.3.6 Changes in Particle Size and Zeta Potential of PWP–Dim Nanoparticles During Storage
3.4 Conclusions
CHAPTER 4 Effect of Ultrasound Treatment on the Physicochemical, Microstructural and Antioxidative Properties of Whey Protein Concentrate Encapsulated 3,3’-Diindolylmethane Nanoparticles
4.1 Materials and Equipments
4.1.1 Materials and Reagents
4.1.2 Instruments and Equipments
4.2 Methodology
4.2.1 Preparation of Nanoparticles
4.2.2 Ultrasound Treatment of WPC–DIM Nanoparticles
4.2.3 Nanoparticles Characterization
4.2.4 Encapsulation Efficiency (EE%)
4.2.5 Rheological Determination
4.2.6 Color and pH Measurement
4.2.7 Transmission Electron Microscopy (TEM)
4.2.8 Freeze Drying
4.2.9 Differential Scanning Calorimetry (DSC)
4.2.10 Fourier Transform Infrared Spectroscopy (FT-IR)
4.2.11 Antioxidant Activity
4.2.12 Statistical Analyses
4.3 Results and Discussion
4.3.1 Effect of Ultrasound Treatment on the Particle Size, Polydispersity Index (PDI) and Zeta Potential of WPC–DIM Nanoparticles
4.3.2 Effect of Ultrasound Treatment on the Encapsulation Efficiency (EE%)of WPC–DIM Nanoparticles
4.3.3 Effect of Ultrasound Treatment on Rheological Properties of WPC–DIM Nanoparticles
4.3.4 Effect of Ultrasound Treatment on Color and pH of WPC–DIM Nanoparticles
4.3.5 Effect of Ultrasound Treatment on Microstructure of WPC–DIM Nanoparticles
4.3.6 Effect of Ultrasound Treatment on Differential Scanning Calorimetry(DSC) of WPC–DIM Nanoparticles
4.3.7 Effect of Ultrasound Treatment on Fourier Transform Infrared (FT-IR)Spectra of WPC–DIM Nanoparticles
4.3.8 Effect of Ultrasound Treatment on Antioxidant Activity of WPC–DIM Nanoparticles
4.4 Conclusions
CHAPTER 5 Clinical Study on Oral Whey Protein Encapsulated DIM in Castrate-resistant Metastatic Prostate Cancer
5.1 Materials and Equipments
5.1.1 Materials and Reagents
5.1.2 Instruments and Equipments
5.2 Methodology
5.2.1 Patients Selection
5.2.2 Study Design
5.2.3 Treatment Protocol
5.2.4 Quality of Life(QoL)
5.2.5 Biochemical Assessment
5.2.6 Statistical Analysis
5.3 Results and Discussions
5.3.1 Anthropometric Measurement of the Patients.
5.3.2 Structure of the EORTC QLQ-PR25
5.3.3 Quality of Life
5.3.4 Effect of the Intervention on PSA Levels of the Patients
5.3.5 Medical Events and Side Effects
5.3.6 Discussion
5.3.7 Conclusions
CHAPTER 6 CONCLUSIONS
6.1 Summary
6.2 Conclusions and Recommendations
参考文献
ANNEXURE I
CONSENT LETTER
ANNEXURE II
Self Introduction and the Scientific Research Achievements Obtained During the Doctoral Degree
致谢
吉林大学;
