声明
List of abbreviation
Chapter 1: Introduction
1.1.1 Introduction
1.1.2Sources of heavy metal(loid)s in the soil environment
1.1.3 Heavy metal(loid)s effects
1.2Veterinary antibiotics
1.2.1Oxytetracycline (OTC)historic use
1.2.2Properties of the oxytetracycline substance
1.2.3Specific uses of the oxytetracycline substance
1.2.4Veterinary antibiotics(VA) in the environment
1.2.5Veterinary antibiotic sorption to soil
1.2.6Environmental effect of veterinary antibiotics
1.2.7Degradation methods and pathways of oxytetracycline
1.2.8Degradation factors of oxytetracycline in composting
1.3The main problems of this research
1.4 Objectives of research
Chapter 2:Changes in Arsenic and Copper bioavailability and Oxytetracycline degradation during the composting process
2.1 Introduction
2.2Materials and methods
2.2.1Composting process and sampling
2.2.2Physiochemical analyses
2.2.3The heavy metal(loid)sextraction
2.2.4OTC extraction and HPLC analysis
2.2.5Germination index
2.2.6Statistical analysis
2.3 Results
2.3.1 Temperature
2.3.2 Physiochemical properties
2.3.3The germination index(GI %)
2.3.4Changes in the As and Cu fractions
2.3.5Bioavailability of As and Cu
2.3.6 OTC degradation during composting
2.3.7Linear regression analysis for the germination index
2.4 Discussion
2.5 Conclusion
Chapter 3 :Microbial community dynamic during composting of animal manures contaminated with arsenic, copper, and oxytetracycline
3.1 Introduction
3.2Materials and methods
3.2.1DNA extraction and bioinformatic static analysis
3.2.2Bioinformatic static analysis
3.3 Results
3.3.1Principal components analysis
3.3.2Species abundance analysis
3.3.3Alpha diversity indices
3.3.4Chao1 index
3.3.5Shannon index
3.3.6Simpson index
3.3.7Redundancy analysis
3.4 Discussion
3.4.1Microbial diversity
3.4.2Microbial composition distribution
3.4.3Redundancy analysis
3.5 Conclusion
Chapter 4 :Conclusion
Conclusion
Innovation points
Recommendations
Further studies are requested to
Publication
参考文献
致谢
Resume
中国农业科学院;
