声明
Abbreviations
Chapter 1 INTRODUCTION
1.1 Mushrooms in Human Cultures
1.1.1 Mushrooms as Food Source
1.1.2 Medicinal Use of Mushrooms
1.1.3 Other Uses of Mushroom in Human Society
1.1.4 Why Choose Shiitake?
1.2 What is Tumor?
1.2.1 Importance of Studying Tumor
1.2.2 How tumors are formed?
1.2.3 Prognosis of Lung Cancer and the Importance of Studying it
1.3 Anti-Tumor Myco-Metabolites and Their Anti-Tumor Mechanisms
1.3.1 Extraction of Bioactive Mushrooms
1.3.2 Isolation and Identification of Myco-metabolites
1.3.3 Safety and Efficacy of Bioactive Compounds
1.4 Structure-Activity Relationship of Metabolites
1.5 Aims and Objectives of This Study
1.5.1 Aims and Objectives
1.5.2 Technical route
Chapter 2 MATERIALS AND METHODS
2.1 Shiitake Extract Preparation
2.1.1 Mushroom Powder Preparation
2.1.2 Extraction of the Mushroom Powder
2.1.3 Evaporation of the Mushroom Extract
2.2 Isolation of Metabolites from Extract
2.2.1 Column chromatography of Extract
2.2.2 Semi-Preparatory High-Performance Liquid Chromatography
2.3 Cytotoxicity Test of Fractions and Isolates
2.3.1 A-549 Cell Culture Preparation
2.3.2 Counting Cultured A-549 Cell
2.3.3 Cytotoxicity Test of Extract, Fraction and Metabolites
2.4 Identification of Compounds Isolated from Shiitake
2.4.1 LC-MS Analysis of Purified Compounds
2.4.2 1H NMR Analysis of Isolated Compound
Chapter 3 RESULT
3.1 Extraction of Shiitake 808
3.2 Isolation and Purification of Cytotoxic Compounds from Shiitake 808
3.3 Identification of Purified Compound
Chapter 4 DISCUSSION
4.1. Myco-metabolites from Shiitake Mushroom
4.2. Cytotoxicity of Shiitake Metabolites
Chapter 5 CONCLUSION
参考文献
Appendix A
Appendix B
致谢
Resume of the Author
中国农业科学院;
