文摘
英文文摘
论文说明：Abbreviation
声明
Chapter Ⅰ: Introducton
1.1 The Bcr-Ab1 tyrosine kinase and generation of chronic myeloid leukemia (CML)
1.2 The roles of Ab1 interactor (Abi) in cells
1.2.1 The discovery of Abi family proteins
1.2.2 Abi interacts with Ab1 and influence the signal transduction regulated by Ab1 tyrosine kinase
1.2.3 Abi could form Eps8-Abi1-Sosl complex involved in Eps8 pathway
1.2.4 The function of Abi family in actin polymerization through interacting with WAVE/WASP family members
ChapterⅡ: Investigation of the role of Abi2 protein in tumor cells
2.1 Material and methods
2.1.1 Cell culture and reagent
2.1.2 In vitro translation assay
2.1.3 Cell cycle synchronization
2.1.4 SDS-PAGE and western blotting
2.1.5 Immunoprecipitation
2.1.6 In vitro phosphorylation Kinase assays
2.1.7 Plasmid constructs
2.1.8 Fluorescence microscopy
2.2 Results
2.2.1 Dysregulation of Abi1 and Abi2 by Bcr-Ab1 in BaF3p185wt cells
2.2.2 Define the sequences in Abi2 that are required for Bcr-Ab1-induced downregulation
2.2.3 Identification of molecular events downstream of Bcr-Ab1/Abi1 signaling
2.2.4 Deferential regulation and function of Abi1 and abi2 isoforms in vivo
2.2.4 Abi2B/A is involved in the regulation of MMP-9 expression in MDA-MB231 cells
2.2.5 Abi is phosphorylated by CDC2 in a cell-cycle dependent fashion
2.3 Discussion
Chapter Ⅲ: Abi1 regulates MMP9 expression and invadopodia formation
3.1 Material and methods
3.1.1 Cells cultures
3.1.2 Transfection
3.1.3 Antibodies and reagents
3.1.4 Immunocytochemistry and fluorescence microscopy
3.1.5 In vitro ECM degradation assay
3.1.6 Cell migration and proliferation assays
3.1.7 Biochemical assays
3.1.8 Real-time quantitative reverse transcription-PCR
3.1.9 In vivo tumorgenesis studies
3.1.10 Statistical analyses
3.2 Results
3.2.1 Abi1 is found in invadopodia in MDA-MB231 cells
3.2.2 Abi1 silencing inhibited invadopodia formation and ECM degradation of MDA-MB231 cells
3.2.3 Abi1 is involved in the regulation of MMP-9 expression in MDA-MB231 cells
3.2.4 Activation of Src tyrosine kinase is essential for the regulation of Id1/MMP-9 expression and formation of invadoodia in MDA-MB231 cells
3.2.5 Abi1 silencing inhibited the Src-Id1 signaling and impaired MDA-MB231 cell migration and proliferation
3.2.6 Knockdown of Abi1 expression markedly slowed tumor growth of MDA-MB231 breast cancer cells
3.2.7 A hypothetical model of the Abi1 signaling that regulates invadopodia formation and the Src pathway in MDA-MB231 breast cancer cells
3.3 Discussion
Conclusions
References
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致谢
