Identification of cis--element and trans--factors functioning in nuclear retention of lncRNA MEG3

目录

声明

Contents

ABSTRACT(English)

摘要

INTRODUCTION

Long noncoding RNA(LncRNA)Introduction

Mechanism of RNA localization

Locaiization of LncRNAs

Importance of lncRNA localization

Quantitative Real Time PCR(qPCR)

Nuclear Retention of lncRNAs

MATElUALS AND METHODS

Constructs and Antibodies

Cell Culture and Transfecfion

Mini Nuclear Extract Preparation

In Vitro RNP Purification

Mass Spectrometry Analysis

RNA Sequencing Analysis(RNA-seq)

RESULTS

Nuclear Localization of MEG3 Reporter Transcript

Identification of nuclear retention element in MEG3

Nuclear Retention of Cytoplasmic RNAs by MEG3 NRE

NRE Associated LncRNPs Purification

Knockdown of U1snRNP Led to accumulation of MEG3 reporter transcripts in cytoplasm

Knockdown of U1snRNP Led to cytoplasmic accumulation of LncRNAs

DISCUSSION

REFERENCES

LITERATURE REVIEW

APPENDIX

ACKNOWLEDGMENTS