文摘
英文文摘
论文说明：List of tables List of Figures 、ABBREVIATIONS
DECLARATION
CHAPTER ONE Introduction
CHAPTER TWO Literature Review
2.1 Characterization and functional of avian TCF3
2.2 Semi-quantitative RT-PCR technique to assess TCF3 expression
2.3 TCF3 expression differential
2.4 Selection depends on gene expression variations
2.5 Single nucleotide polymorphisms (SNPs)
CHAPTER THREE Materials and Methods
3.1 Experimental stock and sample size
3.1.1 Duck breeds
3.1.2 Chicken breeds
3.2 Phenotypic measurements
3.3 Blood samples collection and processing
3.4 Samples collection and preparation
3.4.1 DNA isolation
3.4.2 DNA purification and concentration
3.5 RNA extraction and purification
3.6 Reverse transcription (RT)
3.7 Primer sequences
3.8 Semi-quantitative RT-PCR
3.8.1 Submerged agarose gel electrophoresis (SAGE)
3.8.2 Gel image acquisition and quantitative analysis
3.9 SSCP-PCR protocol
3.9.1 SSCP-PCR reactions
3.9.2 Polyacrylamide gel electrophoresis (PAGE)
3.9.3 Detection of single nucleotide polymorphisms (SNPs)
3.10 Statistical analysis
CHAPTER FOUR Results
4.1 TCF3 expression patterns
4.2 Differential expression of TCF3 in different samples
4.3 Calibration of RT-PCR signals
4.4 Variations of TCF3 relative expression level
4.4.1 Chicken breeds
4.4.2 Duck breeds
4.5 Association of TCF3 relative expression level and phenotypic characters
4.5.1 Chicken breeds
4.5.2 Duck breeds
4.6 Detection of single nucleotide polymorphisms (SNPs)
4.6.1 Single nucleotide polymorphisms（SNPS） confirmation
4.6.2 SSCP-PCR product automated sequencing
4.6.3 Hardy-Weinberg Equilibrium (HWE) analysis
4.7Association of TCF3 relative expression level and SNPs
4.8 The effect of SNPs genotypes and sex upon TCF3 relative expression level
CHAPTER FIVE Discussion
5.1 Expression pattern of TCF3 factors E12/E47
5.2 Monitoring of TCF3 relative expression variations
5.3 TCF3 relative expression and sexual dimorphism
5.4 Association of TCF3 relative expression and body weight characters
5.5 Single nucleotide polymorphisms (SNPs) analysis
5.6Correlation between TCF3 relative expression and SNPs
CHAPTER SIX Summary and Conclusion
References
Acknowledgement
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