CONTENTS
Abstract
Symbols
Chapter 1：General Introduction
1．1 General life cycle of powdery mildews
1．2 Effect of temperature on the development of powdery mildews
1．3 Inheritance of powdery mildew resistance and relation to temperature
1．4 References
Chapter 2：Literature Review
2．1 Markers，populations and map construction
2．1．1 Genetic markers
2．1．2 Mapping population
2．1．3 Map construction
2．2 Statistical methods for QTL mapping．
2．2．1 Single marker analysis
2．2．2 Interval mapping
2．2．3 Composite interval mapping
2．2．4 Multiple interval mapping
2．3 Epistatic and environmental interactions for quantitative trait loci
2．4 QTL mapping in Cucubitaceae species
2．4．1 QTL mapping and linhkage analyses in Cucumber
2．5 Plant defence signalling networks
2．5．1 Mitogen activated protein kinases
2．5．2 Calcium dependent protein kinases
2．5．3 Phospholipid signalling
2．5．4 Reactive oxygen species and nitric oxide
2．5．5 Salieylic acid and systemic acquired resistance
2．5．6 Jasmonic acid and ethylene
2．5．7 Cross talk between signalling pathways
2．6 Biochemistry of plant defence responses
2．6．1 Pathogenesis related proteins
2．6．2 Cell wall fortification
2．6．3 Lipoxygenases
2．6．4 Phytoalexins
2．6．5 Transcription factors
2．7 Reference
Chapter 3：SCAR Marker Linked to Powdery Mildew Resistance for Selection of Near-isogenc Lines in Cucumber．
3．1 Introduction
3．2 Materials and Methods
3．2．1 Plant materials
3．2．2 Disease resistance test
3．2．3 Marker analysis
3．2．4 Screening and markers verifying
3．2．5 Statistical analysis
3．2．6 Excision and cloning procedures
3．2．7 Selection of resistant NILs using SCAR markers
3．3 Resuits
3．3．1 Markers screening
3．3．2 SCAR designing and sequencing
3．3．3 Analysis of SCAR and resistance gene linkage
3．3．4 MAS in resistant NILs using SCAR markers
3．4 Discussion
3．5 Conelusion
3．6 References
Chapter 4：Mapping quantitative trait loci for powdery mildew resistance in cucumber using SRAP and ISSR markers．
4．1 Introduetion
4．2 Materials and Methods
4．2．1 Plant materials
4．2．2 Disease resistance test
4．2．3 DNAextraction and molecular marker analysis
4．2．4 QTL analysis
4．3 Resuits
4．3．1 Powdery mildew investigation
4．3．2 ISSR and SRAP markers analyses
4．3．3 Linkage map and marker segregation
4．3．4 QTL analysis
4．4 Discussion
4．5 Conclusion
4．6 References
Chapter 5：Expression of WRKY and MYB Genes during infection with powdery mildew in cucumber．
5．1 Introduction
5．2 Materials and Methods
5．2．1 Plant and fungal materials
5．2．2 RNA isolation and reverse transcription-PCR
5．2．3 Quantitative real-time PCR and gene expression analysis
5．3 Resuits
5．4 Discussion
5．5 Conclusion
5．6 References
6．Conclusion
7．Acknowledgement
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