声明
论文说明
List of Contents
ABSTRACT
摘要
Abbreviation
Introduction
References
Chapter 1 Literatu re Review The role of thyroid hormones in ovarian follicular development and function
1．1 The anatomy and functions of the thyroid gland
1．2 Regulation of T3 and T4
1．3 The synthesis of thyroid hormones
1．4 The mechanism of thyroid hormones
1．5 Thyroid hormones transportation
1．6 The hypothalamie pituitary thyroid axis(HPT)and female reproductive system
1．7 Effects of thyroid hormones in ovarian follicular development
1．8 Hyperthyroidism and infertility
1．9 Hypothyroidism and infertility
1．10 Effects of thyroid hormones in ovarian follicular development and function in hyper and hypothyroid neonatal and immature rats
1．11 Effects of thyroid hormones on the ovarian follicular development and function in hyper and hypothyroid adult rats
References
Chapter 2 Roles of Nitric Oxide in ovarian development and function in hyper and hypothyroid rats
2．1 General characteristics of NOS
2．2 The NOS molecular structu re and biosynthesis
2．3 NOS gene catalytic mechanism and function
2．4 Nitric Oxide regulation and inhibition
2．5 Nitric Oxide and ovarian funotion
2．6 Effects of nitric oxide activity in ovarian follicular development and function in hyper and hypothyroid rats
References
Chapter 3 Roles of thyroid hormones in ovarian follicular development in neonatal and immature rats
3．1 Introduction
3．2 Materials and Methods
3．2．1 Animals
3．2．2 Experimental design
3．2．3 Tissue preparation
3．2．4 Radioimmunoassay(RIA) for triiodothyronine(T3)，thyroxine (T4) and 17β-estradiol(E2)
3．2．5 Histologic examination
3．2．6 Immunohistochemistry
3．2．7 Measurement of NOS activity
3．2．8 Statistical analysis
3．3 Results
3．3．1 Body weights of control，and induced hyperthyroid and hypothyroid rats
3．3．2 Serum hormone concentrations in control,layper and laypo rats
3．3．3 Ovarian histologic and morphometric analyses
3．3．4 Immunohistochemicai staining of TRα 1 and NOS isoforms in the ovary of neonatal rats
3．3．5 Measurement of NOS activity
3．4 Discussion
References
Chapter 4 Nitric oxide and thyroid hormone receptor alphalcontribute to ovarian follicular development in immature hyper-and hypo-thyroid rats
4．1 Introduction
4．2 Material and Methods
4．2．1 Animals and experimental design
4．2．2 Radioimmunoassay of hormones
4．2．3 Protein homogenate preparation and hormone management
4．2．4 Histologic examination and follicle counts
4．2．5 Immunohistochemicai Analysis
4．2．6 Measurement of ovarian contents of NO
4．2．7 Measurement of ovarian NOS activity
4．2．8 Statistical analysis
4．3 RESULTS
4．3．1 Serum hormone measurements
4．3．2 Histologic examination offollicular development
4．3．3 Immunohistochemical localizatioil of NOS isoforms and TRα1
4．3．4 Measurement of ovarian contents of NO
4．3．5 Measurementofovarlan actlvity of NOS
4．4 DISCUSSION
Referenees
Chapter 5 Effects of thyroid hormones in puberty onset and estrous cyclicity of young adult rats
5．1 Introduction
5．2 Material and Methods
5．2．1 Ethies statement
5．2．2 Reagents
5．2．3 Animals and condition
5．2．4 Experimental design
5．2．5 Tissue preparation
5．2．6 Observation of vaginal opening days and estrous cyclicity
5．2．7 Determination of serum coneentrations of hormones
5．2．8 Histology and follicle counting
5．2．9 Protein extraction and western blot analysis
5．2．10 lmmunohistoehemistry
5．2．11 Statistical analysis
5．3 Results
5．3．1 Boay and ovarian weights of control and induced hyperthyroid and hypothyroid rats
5．3．2 Vaginal opening (VO) days，puberty onset and estrous cycles in control,hyperthyroid and hypothyroid rats
5．3．3 Serum concentrations of hormones in control,hyperthyroid and hypothyroid rats
5．3．4 Ovarian histologic and morphometric examination
5．3．5 TRα1 involvement in the process of follicular development
5．3．6 Immunohistochemical detection of TRα 1 and TRα／β in the ovaries of aduR rats
5．4 DISCUSSION
Conclusions
Reference
Conclusions
Innovation
Future Research Openings
List of Published，Submitted and Under Preparation Papers
Acknowledgements
Appendix
