环境因素对螺旋藻的生长和生化成分影响的分析

摘要

螺旋藻被广泛应用于人们生活的方方面面，如卫生、食品和化妆品行业等。本论文通过改变不同的物理和化学因素水平，即不同pH，纳米氧化镁(MgO)，氮源，磷源，碳源，盐浓度和生长培养基等，对螺旋藻的生物量，叶绿素和蛋白质的生产条件进行优化，达到优化生物量的目的。实验过程中螺旋藻的生长温度30±2℃，光强为4400lux，光/暗周期为14∶10 h。本论文探讨了不同环境因素水平对螺旋藻培养生理生化指标的影响，主要结果如下:
　　培养基中pH=9时，螺旋藻的干重、蛋白质和叶绿素a含量最高，分别为7.83±0.29 g/L，1.34±0.12mg/mL，18.64±0.06μg/mL。当MgO的浓度为0.8mM时，最大干重、蛋白质和叶绿素含量分别为3.48±3.48 g/L，3.44±0.312 mg/mL，4.80±0.12μg/mL。以NaNO3作为氮源时，获得螺旋藻的干重为2.24±0.13 g/L，而蛋白质和叶绿素a含量分别为3.24±0.30 mg/mL和2.53±0.24μg/ml。以K2HPO4作为磷源时，获得最大的干重为2.08±0.17g/10ml，蛋白质和叶绿素含量分别为2.93±0.06mg/ml，2.52±0.21μg/ml。NaHCO3作为碳源时，最高干重为1.18±0.02 g/L，蛋白质含量为2.97±0.10mg/ml，叶绿素a含量为1.15±0.11μg/ml。采用通用Zarrouk培养基时培养时，干重为3.48±0.34 g/L，蛋白质含量为5.26±0.41mg/ml，叶绿素a含量为5.18±0.43μg/ml。改变培养基的盐浓度，发现当盐浓度为2.5％时，获得最大干重为1.44±0.06 g/L，蛋白质含量为117.77±2.00μg/ml，叶绿素a含量为1.86±0.13 mg/L，且获得了最高细胞生长速率0.22±0.01μM/天。本研究结果可为大规模生产高蛋白质含量、极具发展前途的健康食品螺旋藻提供理论依据。

目录

声明

DEDICATION

Table of content

List of abbreviations

ABSTRACT

摘要

CHAPTER 1 INTRODUCTION

1．1 Background Information

1．2 Statement of the problem

1．3 General objective

1．4 Specific objectives

1．5 Hypothesis(Ho)

1．6 Significance of the study

1．7 Scope of the study

CHAPTER 2 LITERATURE REVIEW

2．1 Introduction

2．2 General characteristics of Spirulina

2．2．1 Morphology and taxonomy

2．2．2 Natural habitat，source and growth

2．2．3 Biochemical composition

2．2．4 Proximate composition

2．2．5 Chelating of toxic minerals(neutralization of toxic minerals)

2．3 Cultivation and Production of Spirulina

2．3．1 Natural production

2．3．2 Laboratory cultivation

2．4 Small-scale commercial production of Spirulina

2．5 Commercial and mass cultivation

2．6 Examples of production around the world

2．7 Harvesting and processing

2．8．Products，gses and benefits for humans，fish and other animals

2．8．1．Spirulina and its use by humans

2．8．2 Spirulina and agriculture

2．8．3 Spirulina and aquaculture

2．9．Recent development and future outlook

2．9．1 Gene manipulation

2．9．2 Plant growth regulators

2．9．3 Strain development and improvement

2．9．4 Expanding production to new countries

2．9．5 Potential use as a nutritional supplement in humanitarian emergencies

CHAPTER 3 MATERIALS AND METHODS

3．2 Culture media and growing conditions

3．3 Cultivation

3．4 Biomass yield

3．5 Biochemical analysis

3．6 Statistical analysis

CHAPTER 4 RESULTS AND DISCUSSION

4．1 Effect of different pH levels on biomass production of Spirulina

4．2 Effect on different concentrations of nano MgO to Spirulina biomass production

4．3 Effect of different nitrogen，phosphorus and carbon sources to biomass production of Spirulina

4．4．Effect of different salinities to biomass production of Spirulina

4．5 Biomass production of Spirulina using different liquid media

CHAPTER 5 CONCLUSIONS AND RECOMMENDATIONS

5．1．CONCLUSIONS

5．2．RECOMMENDATION

REFERENCES

Acknowledgement

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