Effect of Dietary Bamboo Charcoal Supplementation on the Growth and Physiological Performance,Disease Resistance and Intestinal Bacteria Community Structure of Juvenile Common Carp(Cyprinus Carpio L.)

目录

声明

Table of Contents

ABSTRACT

摘要

CHAPTER 1 Background，Literature Review and Justification

1．1General Overview of Aquaculture Production

1．2 The Common Carp(Cyprinus carpio L．)and its Production in China

1．3 Factors that Affect Growth，Health Status and Overall Performance of Fish

1．3．1 Temperature

1．3．2 Dissolved Oxygen

1．3．3 Ammonia

1．3．4 Nutrition

1．4 Applications of Feed Additives in Aquaculture

1．4．1 Phytogenic feed additives

1．4．2 Probiotics

1．4．3Prebiotics

1．4．4 Immunostimulants

1．4．5 Organic acids

1．5 Bamboo Charcoal as a Potential Feed Supplement

1．5．1Use of Charcoal as a Dietary Feed Supplement

1．6 Justification of the Study

1．7 Objectives of the Study

CHAPTER 2 Effect of Dietary Bamboo Charcoal Supplementation on the Growth Performance，Serum Biochemical Indices，Intestinal Histomorphology，Muscle Fatty Acid Composition and Resistance of Juvenile Common Carp(Cyprinus carpio L．)to Aeromonas hydrophila Infe

2．1 Introduction

2．2 Materials and Methods

2．2．1 Experimental Fish and Rearing Facility

2．2．2 Experimental Diets

2．2．3 Feeding Trial

2．2．4 Ethical Statement

2．2．5 Sampling Methods

2．2．6 Blood Sampling and Preparation

2．2．7 Intestinal Histo-mophological Analysis

2．2．8 Muscle Fatty Acid Composition Analysis

2．2．9 Aeromonas hydrophila Challenge Test

2．2．10 Statistical Data Analysis

2．3 Results

2．3．1 Effect of Dietary BC on Growth Performance

2．3．2 Effect of Dietary BC on Serum Biochemical Parameters

2．3．3 Effect of Dietary BC on Intestinal Morphology

2．3．4 Effect of Dietary BC on Muscle Fatty Acid Composition

2．3．5 Effect of Dietary BC on Resistance of Juvenile Common Carp to Aeromonas hydrophila

2．4 Discussion

2．5 Conclusion

CHAPTER 3 Effect of Dietary Bamboo Charcoal Supplementation on the Intestinal Microbiota Community of Juvenile Common Carp (Cyprinus carpio L．)

3．1 Introduction

3．2 Materials and Methods

3．2．1 Experimental Fish and Rearing Facility

3．2．2 Experimental Diets

3．2．3 Feeding Trial

3．2．4 Ethical Statement

3．2．5 Sampling Methods

3．2．6 DNA Extraction and Purification

3．2．7 PCR Amplification，Purification and Pyrosequencing

3．2．8 Statistical and Bioinformatics Analysis

3．3 Results

3．3．1 General Analyses of the Deep Sequencing-Derived Dataset

3．3．2 Composition of Bacterial Community

3．3．3 Core Intestinal Microbiota

3．3．4 Relationships between gut bacterial communities in the BC supplemented and non-supplemented common carp bacterial communities

3．3．5 Linear discriminant analysis(LDA)effect size(LEfSe)

3．4 Discussion

3．5 Conclusion

General Conclusions

REFERENCES

APPENDIX

ACKNOWLEDGEMENTS

SEMINARS AND SYMPOSIA ATTENDED