文摘
英文文摘
Abbreviations
Chapter 1: Review of literature
1.1. Cotton fiber development
1.1.1 Fiber development mechanism
1.1.2 Approaches for the improvement of cotton fiber
1.2 Factors affecting cotton growth and development
1.2.1 Abiotic factors
1.2.2 Biotic factors
1.3 Thaumatin-like protein (TLP) gene: A member of PR family
1.3.1 Role of Pathogenesis-related proteins in plant defense
1.4 Functional study of a gene
1.4.1 Over-expression
1.4.2 RNA silencing
1.5 Genetic engineering of commercially important plants
1.5.1 Genetic engineering of tobacco
1.5.2 Genetic engineering of cotton
1.6 Mechanism of Agrobacterium-mediated transformation
1.6.1 Agrobacterium: 'Natural' genetic engineering
1.6.2 Binary vectors: two from one
1.6.3 Steps involved in Agrobacterium-mediated transformation
1.7 Aims, strategies and overview of the present study
1.7.1 Aims of the study
1.7.2 Approaches/strategies to accomplish the intended objectives
1.7.3 An overview of the present study
Chapter 2: Materials and methods
2.1. Study of GbTLP1 gene in tobacco
2.1.1 Vector construction
2.1.2 Transformation and growth of tobacco
2.1.3 DNA extraction, PCR and Southern blot analysis
2.1.4 RNA extraction, RT-PCR and Quantitative Realtime PCR analysis
2.1.5 Disease resistance evaluation
2.1.6 Salt and oxidative stress assay
2.1.7 Whole plant drought tolerance test
2.1.8 Bioinformatics studies of GbTLP-1 and other proteins of the same family
2.1.9 Statistical analysis
2.2 Study of GbTLP gene in cotton
2.2.1 Vector construction
2.2.2 Transformation and growth of transgenic cotton
2.2.3 DNA extraction and PCR for the selection of positive plants
2.2.4 Standard high volume instrumentation (HVI) measurements
2.2.5 Future plan of this study
Chapter 3: Results
3.1 Characteristic features of GbTLP1
3.2 Role of GbTLP1 in secondary cell wall deposition
3.3 Results obtained by the transformation of tobacco
3.3.1 Transformation and regeneration of transgenic tobacco plants
3.3.2 Integration and higher GbTLP1 expression in transgenic tobacco
3.3.3 Disease resistance in GbTLP1 transgenic plants
3.3.4 Role of GbTLP1 transgenic plants under salinity and oxidative stress
3.3.5 Enhanced tolerance of transgenic plants under drought stress
3.4 Results obtained by the genetic transformation of cotton
3.4.1 Transformation and regeneration of transgenic cotton plants
3.4.2 Selection of positive transgenic cotton plants
3.4.3 Standard high volume instrumentation (HVI) measurements of fiber properties
3.4.4 Expected results and conclusion
Chapter 4:Discussion
Chapter 5:Major findings and future recommendations
5.1 Major Findings
5.2 Future Recommendations
References
Related papers
Acknowledgements
CURRICULUM VITAE(C.V.)
