声明
论文说明
Table of Contents
Abstract
摘要
Chapter 1-Literature review
1．1 Introduction
1．2 The developing goat industry in China and the importance of parasites
1．3 Key parasitic helminths of goats and their biology
1．4 The pathogenesis of diseases and clinical manifestation of parasitic helminths infections／diseases
1．4．1 Haemonchus contortus
1．4．2 Ostertagia species
1．4．3 Teladorsagia circumcincta
1．4．4 Trichostrongylus colubriformis
1．4．5 Cooperia species
1．4．6 Bunostomum trigonocephalum
1．4．7 Oesophagostomum columbianum
1．4．8 Oesophagostomum asperum
1．4．9 Trichuris species
1．4．10 Paramphistomum cervi
1．4．11 Fischoederius elongatus
1．4．13 Homalogaster paloniae
1．4．14 Fasciola hepatica
1．4．16 Dicrocoelium dendriticum
1．4．17 Eurytrema pancreaticum
1．4．18 Taenia hydatigena
1．4．19 Moniezia expansa
1．5 Diagnosis of parasitic helminths infections／diseases of goats
1．5．1 Diagnostic methods based on clinical symptoms of animals
1．5．2 Faecal egg counts
1．5．3 Larval culture
1．5．4 Immunological methods
1．5．5 Post-mortem diagnosis
1．5．6 Molecular methods
1．6 Epidemiology of parasitic helminths infections／diseases of goats
1．7 Current treatment and control strategies in China
1．7．1 Vaccine
1．7．2 Anthelmintics
1．7．3 Management
1．8 Drug resistance in parasitic helminths of goats in China
1．8．1 Mechanism of drug resistance
1．8．2 Diagnostic methods for detecting drug resistance
1．8．3 Current drug resistance in China
1．9 Conclusions from the literature review，and aims of the thesis
Chapter 2-Survey of parasitic helminths of goats along the Han River in Hubei Province，China
2．1 Introduction
2．2 Materials and methods
2．2．1 Study area，animals and sample collection
2．2．2 Conventional faecal examination
2．2．3 Molecular diagnosis
2．3 Results
2．4 Discussion
Chapter 3-Investigation of helminths of goats along the Dabie Mountain in Hubei Province
3．1 Introduction
3．2 Materials and methods
3．2．1 Study area
3．2．2 Post mortem examination
3．2．3 Isolation of genomic DNA from parasites
3．2．4 Molecular identification of gastrointestinal nematodes
3．2．5 Molecular identification of trematodes
3．3 Results
3．4 Discussion
Chapter 4-Genetical characterization of Fischoederius elongatus using mitochondrial genome sequencing
4．1 Introduction
4．2 Materials and methods
4．2．1 Parasite collection and DNA isolation
4．2．2 Amplification and sequencing of F．elongatus mt genome
4．2．3 Sequence analyses
4．2．5 Phylogenetic analysis
4．3 Results
4．3．2 Protein-coding genes
4．3．3 Transfer RNA and ribosomal RNA genes
4．3．5 Nucleotide variability between F．elongatus and P．cervi
4．3．6 Genetic relationships
4．4 Discussion
Chapter 5-Comparative analysis of the full mitochondrial genome of Gastrothylax crumenifer
5．1 Introduction
5．2 Materials and methods
5．2．1 Parasites and DNA isolation
5．2．2 Amplification and sequencing of G．crumenifer mt genome
5．2．3 Sequence analyses
5．2．4 Phylogenetic analysis
5．3 Results
5．3．1 Characteristics of the G．crumenifer mt genome
5．3．2 Protein-coding genes
5．3．3 Transfer RNA genes，ribosomal RNA genes and Non-coding regions
5．3．4 Nucleotide variability
5．3．5 Phylogenetic analyses
5．4 Discussion
Chapter 6-Characterization of the complete mitochondrial genome sequence of Homalogaster paloniae
6．1 Introduction
6．2 Materials and methods
6．2．1 Parasites and DNA extraetion
6．2．3 Assembly，annotation and bioinformaties analyses
6．2．5 Phylogenetic analysis
6．3 Results
6．3．1 Genome content and organization
6．3．2 Annotation of H．paloniae mt genome
6．3．3 Comparative analyses of the mt genomes
6．3．4 Nucleotide variability
6．3．5 Phylogenetie analyses
6．4 Discussion
Chapter 7-Development and evaluation of a loop-mediated isothermal amplification(LAMP)assay for the detection of Haemonchus contortus in goat fecal samples
7．1 Introduction
7．2 Materials and methods
7．2．1 Parasites and genomie DNA isolation
7．2．2 LAMP primer design
7．2．3 LAMP reaction
7．2．4 PCR assay
7．2．5 Assessment of nonspecific amplifcation and potential inhibition
7．2．6 Statistical analysis
7．3 Results
7．3．1 Species identification
7．3．2 Sensitivity of LAMP method
7．3．3 Specificity of LAMP method
7．3．4 Assessment of nonspecific amplification and potential inhibition
7．3．5 Clinical sample detection
7．4 Discussion
Chapter 8-An investigation of anthelmintic resistance in worms of goats along the Han River in Hubei Province
8．1 Introduction
8．2 Materials and methods
8．2．1 Study area
8．2．2 Animal grouping
8．2．3 Faecal egg count reduction test(FECRT)
8．2．4 Post-mortem examination
8．2．5 Deworming assays
8．3 Results
8．4 Discussion
Chapter 9-Detection of anthelminfic resistance in gastrointestinal nematodes of Dabieshan Black Goats in Luotian，Hubei Province
9．1 Introduction
9．2 Materials and methods
9．2．1 Experimental design
9．2．2 Faecal egg count reduction test
9．2．3 Statistical analysis
9．2．4 Deworming assays
9．2．5 Post-mortem examination
9．3 Results
9．4 Discussion
Chapter 10-General discussion
Conclusion
References
Acknowledgements
Curriculum vitae
