声明
摘要
ABSTRACT
CONTENTS
ChapterⅠ Introduction
1．1 Studies of chloroplast genomes
1．1．1 Review ofchloroplast genomes
1．1．2 Chloroplast genome size and features
1．1．3 The methods to obtain chloroplast genome data
1．2 Molecular evolutionary research
1．3 Studies of Lauraceae
1．3．1 Characteristics of Lauraceae
1．3．2 Use of Lauraceae
1．3．3 Challenges in Lauraceae
1．3．4 Morphological studies of Lauraceae
1．3．5 Molecular studies of Lauraceae
1．4 Studies of Neolitsea
ChapterⅡ Materials and methodology
2．1 Scientific research foundation
2．1．1 Research and research environment
2．1．2 Scientitic research work
2．1．3 Collaboration requirements
2．2 Experimental materials reagents and equipment
2．3 Scientific research methods
2．3．1 The Strategy of the Whole chloroplast Genome
2．3．2 Chloroplast extraction method
2．3．3 Library construction and sequencing
2．3．4 Assembly of chloroplast Genomic sequences
2．3．5 Assembly of extended nuclear ribosomal cistrons regions
2．4 Bioinformatics analysis method
ChapterⅢ Annotation and evolution in chloroplast genome of Neolitsea
3．1 Basic features in chloroplast genome of Neolitsea
3．2 Chloroplast genome characteristics in Neolitsea
3．3 Repeat and SSR analyses
3．4 Summary
ChapterⅣ Comparison of chloroplast and phylogenetic analyses in Neolitsea
4．1 Comparison of different chloroplast genome in Neolitsea
4．2 Phylogenetic Analysis of Neolitsea
4．3 Summary
ChapterⅤ Discussion and prospects
Prospects
References
Additional appendixs
Acknowledgement
Publication of papers
