Measurement System for Local Electric Impedance of Gel in Cell with Vibrating Electrode

摘要

A new methodology to measure local electric impedance of gel in a cell has been proposed, and a measurement system has been designed with a vibrating electrode. The vibration of electrode was generated with the piezoelectric actuator, and controlled with an electric oscillator. The manufactured system was evaluated in the experiment with endothelial cells in vitro. The endothelial cells were exfoliated from the descending aorta of a rat, and cultured on the hydrophilic modified surface of silicon rubber. One of the electrodes was stuck into the cell under the microscope, and vibrated at the frequency of 100 Hz. Another electrode was dipped into the culture medium, and fixed in the close vicinity of the cell. The output signal was amplified and the frequencies were analyzed. The experimental results show that the local impedance in the order of 0.1 kilo-ohm was calculated from the amplitude component of output signal at 100 Hz.