Hydrogen Production Using Enterobacter Cloacae DM11 with EnhancedYield and Improved Energy Recovery

摘要

Fermentative production of hydrogen by mutated strain Enterobacter cloacae DM11, a locallyisolated facultative anaerobic bacterium, was studied in batch and continuous process using bothfree-suspended and immobilized whole cell cultures. The effects of some important physico-chemicalparameters such as initial substrate concentration, medium pH, temperature, andmedium composition were analyzed with respect to maximum hydrogen productivity. The rate of hydrogen production varied from 26 to 273 mmol.l ?1 h ?1 in immobilized system with differentconcentration of glucose in the feed. Overall material and energy balance of the process weremade on the basis of 3 g l ?1 glucose used as feed in a batch of 20 liter. An energy analysis of thesystem describing the percent conversion of energy in to different forms reveals that around38.3% energy could be recovered in the form of gaseous hydrogen using glucose as a substrate.This is almost three fold higher than the values reported in literature for other biohydrogenationprocess.