Rod Enterobacter spheroplasts Lee Death RV uptake hydrogenase with improved hydrogen light production efficiency (Hup) isolation of deficient strain

摘要

PROBLEM TO BE SOLVED: To provide a new mutated strain of Rhodobacter spaeroides RV used for a hydrogen production, having an improved efficiency in a photoproduction of hydrogen, not capable of exhibiting a hydrogenase uptake activity and imparted with a deposit number CBS100805. ;SOLUTION: This uptake type hydrogenase-lacking strain of Rhodobacter spaeroides is a new mutated strain of Rhodobacter spaeroicies RV, incapable of exhibiting a hydrogenase uptake activity, imparted with a deposit number CBS100805, having an improved efficiency in the photoproduction of hydrogen and used for a fermentation method, etc. for producing hydrogen. The new mutated strain is obtained by inserting a genomic cassete (an interposon) encoding a resistance toward an antibiotic material, Trimethoprim into a gene encoding a large subunit of an uptake type hydrogenase enzyme for inactivating a wild type synthase gene of the Rhodobacter spaeroides RV, then culturing in a medium added with Trimethoprim and selecting strains subjected to a mutation.;COPYRIGHT: (C)1999,JPO