Ultrasensitive Detection of DNA Hybridization by Single Silver Nanoparticle Counting

摘要

Detection of specific DNA sequences is extremely important in the fields of gene analysis, clinical disease diagnosis, forensic applications, tissue matching research, bioterrorism prevention and a variety of other biochemical studies. A number of methods of DNA detection have been developed including optical, chemiluminescence, surface plasmon resonance Raman spectroscopy, hyper-Rayleigh Scattering (HRS), colorimetry and electrochemistry. In order to improve sensitivity and high-throughput, recently, great developments in laser-based detection of fluorescent molecules have made it possible. Such as fluorescence correlation spectroscopy (FCS), fluorescence cross correlation spectroscopy (FCCS), laser-induced fluorescence polarization (LIFP), single molecule fluorescence resonance energy transfer (smFRET), single-molecule two-color coincident detection assay, et al.