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Gold nanoparticles: A Novel Application of Spectral Imaging in Proteomics - Preliminary Results

机译:金纳米颗粒:蛋白质组学中光谱成像的新应用-初步结果

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摘要

The intense research in proteomics is demanding for fast, reliable and easy-to-use methods in order to study the proteome. In this proceeding we report the development of such a novel research tool based on spectral imaging and Resonance Light Scattering gold particles. This method will allow the study of DNA-protein interactions. We suggest a broad range of applications: the screening of proteins binding to a specific DNA sequence, the analysis of binding affinities between protein and DNA, and the investigation of the influence of environmental conditions on the binding. We will explain the principle, first experiments and first results based on Brownian motion.
机译:为了研究蛋白质组,对蛋白质组学的深入研究需要快速,可靠和易于使用的方法。在此过程中,我们报告了基于光谱成像和共振光散射金颗粒的这种新型研究工具的开发。这种方法将允许研究DNA蛋白质相互作用。我们建议广泛的应用范围:筛选与特定DNA序列结合的蛋白质,分析蛋白质与DNA之间的结合亲和力,以及研究环境条件对结合的影响。我们将解释基于布朗运动的原理,首次实验和初步结果。

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