Gold nanoparticles: A Novel Application of Spectral Imaging in Proteomics - Preliminary Results

摘要

The intense research in proteomics is demanding for fast, reliable and easy-to-use methods in order to study the proteome. In this proceeding we report the development of such a novel research tool based on spectral imaging and Resonance Light Scattering gold particles. This method will allow the study of DNA-protein interactions. We suggest a broad range of applications: the screening of proteins binding to a specific DNA sequence, the analysis of binding affinities between protein and DNA, and the investigation of the influence of environmental conditions on the binding. We will explain the principle, first experiments and first results based on Brownian motion.