Influence Of Heat Treatment On Biological Properties Of Chitosan Toward Vascular Cells In Vitro

摘要

Chitosan, the deacetylated product of chitin, has known promise as one of structural biocompatible biomaterials for a number of tissue engineering applications. It is well known that fibroblasts strongly adhere to the chitosan film and chitosan inhibits the cell proliferation although chitosan is not cytotoxic. We also reported that the rigorous heat treatment stimulated the cell growth of human dermal fibroblasts(HDFs) on the heat treated chitosan. Since heat treated chitosan appears to be a good candidate for soft tissue regeneration, it seemed interesting to investigate possible interactions between heat treated chitosan and vascular cells, such as endothelial cells, smooth muscle cells(SMCs), and HDFs. As an attempt to investigate bio-compatibility of the chitosan for an effective vascular graft, we investigated the effect of duration of heat treatment on the biological properties of chitosan films, and evaluated their cell binding capacity and cell proliferation based on MTT assay towards vascular cell types.