Carbonyldiimidazole Immobilization of an Extracellular Matrix Protein Mixture onto poly(HEMA) and Decellularized Esophageal Tissue

摘要

The ESCA data shows values that are consistent with the desired surface chemistries. It would be expected that the nitrogen compositions would be similar for laminin and Matrigel~® because Matrigel~® is comprised mainly of laminin. The REEC adhesion assay shows that cells adhere in higher numbers to the CDI immobilized Matrigel~® and laminin surfaces compared to the gelled Matrigel~®. Thus, CDI immobilization of Matrigel~® leads to a more attractive substrate for REEC growth than gelled Matrigel~®. Adhesion was highest yet on collagen I. This might be expected because collagen I is the major ECM component underneath the basement membrane. In a wound healing situation, it might be necessary for epithelial cells to attach quickly to the underlying ECM collagen and immediately start to remodel it with their own basement membrane proteins. This would explain the quick adhesion, but further tests will be done in order to determine the long term effects of these immobilized proteins. Future ToF-SIMS analysis will give insight into actual fragments of protein left on the surface after immobilization. The idea of immobilizing a mixture of proteins using CDI chemistry is valuable by itself, but the conjunction of that technique with the decellularizing process should lead to an exciting new material.