Inhibitory effect of zinc ions in zinc-containing p-tricalcium phosphate on function of matured osteoclasts

摘要

Reducing stability of bone-implant interface due to aseptic bone loss at the interface is a major problem for orthopedic implants for a long term. The long-term objective of this study is to develop calcium phosphate biomaterials incorporating optimum levels of magnesium (Mg), zinc (Zn) and fluoride (F). These ions have been associated with bone formation, biomineralization and osteoporosis therapy. The results of the previous study suggested that osteoclastic bone resorption at the bone-implant interface was inhibited by Zn in the ZnTCP316. The purpose of the present study is to clarify whether ZnTCP modulates resorption activity of mature osteoclasts or not. In this point of view, we examined formation of actin rings, number of apoptosis cells, expression of marker enzymes and the resorption function of purified mature osteoclasts on ZnTCP in vitro.