Engineering the Homogeneity of Embryonic Stem Cell Microenvironments to Direct Cellular Differentiation

摘要

Significant differences in the size and shape of differentiating EBs were observed between hanging drop, static suspension and rotary suspension methods. Rotary suspension EBs were more uniform in size and shape, similar to hanging drop EBs, than static suspensions and also produced a greater yield of EBs than static suspension cultures. The apparent correlation between the spatial homogeneity of EBs and cell differentiation supports the notion that improving the homogeneity of the stem cell microenvironment can direct the fate and function of stem cells.