Specific cytokines released by monocytes cultured on a degradable polyurethane (D-PHI) influence VSMC response

摘要

Statement of Purpose: Following the implantation of a biomaterial, monocytes play a critical role directing the subsequent cellular and wound healing response [1]. These effects are orchestrated through a combination of monocyte-released factors [2] and direct cell-cell contact between monocytes and other cell types [3]. Previous work evaluating the use of a degradable polar hydrophobic ionic polyurethane (D-PHI) for vascular tissue engineering applications indicated its ability to support both an anti-inflammatory monocyte state [4] while also supporting growth and a contractile vascular smooth muscle cell (VSMC) phenotype [5]. Subsequent work with monocytes in co-culture with VSMCs showed enhanced VSMC growth and infiltration into porous D-PHI scaffolds relative to VSMCs alone [5]. The present work aims to determine if there is a defined relationship between D-PHI-specific interactions with monocytes and the cytokines they subsequently release, which could in turn promote VSMC growth and migration.