Evaluation of Bio-mimic Collagen Ⅱ/HA copolymer as scaffold for Chondrocytes

摘要

The preliminary results demonstrated that collagen-g-HA copolymer was synthesized by the current process. The existence of the reducing ends in the modified HA was confirmed by H1-NMR. From AFM and TEM observations, the modified HA has bonded onto the collagen type Ⅱ fiber by reacting between with collagen Ⅱ-NH2. Chondrocytes cultured in the copolymer in vitro could maintain their phenotype in vitro culture. In this study, we fabrication a bio-mimic collagen Ⅱ/HA copolymer and confirm it can sustain the phenotype for three weeks at least. The feasibility of this copolymer is still under evaluation for application on articular repair.