Adhesive Signature-based, Label-free Isolation of Human Pluripotent Stem Cells

摘要

In summary, we demonstrate significant differences in focal adhesion assembly and adhesion strength between undifferentiated hPSCs and fibroblastic cells. This distinct 'adhesive signature' of hPSCs was exploited to rapidly (<10 min) and efficiently isolate undifferentiated cells as intact colonies from fibroblasts and spontaneously differentiated cells via controlled fluid forces using microfluidics. hPSCs, irrespective of source, passage number, and feeder-free matrix, were isolated in a label-free fashion and enriched to > 98% purity without adversely affecting the phenotype or karyotype of the pluripotent cells.