Surface Plasmon Resonance Sensors for Detection of Survival of Motor Neuron Protein

摘要

Surface Plasmon Resonance (SPR) spectroscopy is a potentially valuable tool for measuring protein-protein interactions and protein levels in vitro and in vivo. Fiber-optic based SPR allows for rapid quantitative measurement of disease markers such as the truncated (exons 1-7) survival of motor neuron (SMN) protein. Unlike micro fluidics-based SPR systems, sample loss is eliminated in fiber-optic SPR and the small size of the fiber optic probes (400μm or smaller) facilitates the potential for use in vivo. Recombinant SMN protein overexpressed in E. Coli as well as native SMN from cultured HeLa cells has been successfully measured using fiber-optic SPR.